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1996 Fiscal Year Final Research Report Summary

Fish cell physiology in improved and advanced culture technique : suspension, non-serum, and extracellular matrix-dependent cultures

Research Project

Project/Area Number 06454100
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTOKYO UNIVERSITY OF FISHERIES

Principal Investigator

HASOBE Masahide  TOKYO UNIVERSITY OF FISHERIES.DEPARTMENT OF AQUATIC BIOSCIENCES,ASSOCIATED PROFESSOR, 水産学部, 助教授 (10218464)

Project Period (FY) 1994 – 1996
KeywordsFISH CELLS / SUSPENSION CULTURE / STATIC-SUSPENSION CULTURE / NON-SERUM CULTURE / EXTRACELLULAR MATRIX / TYPE 1 COLLAGEN / CHSE-ss / CHSE-ns
Research Abstract

Because of the difficulty that advanced method of fish cell culture such as static-suspension, non-serum, and extracellular matrix-dependent culture method, we examined the characteristics of mainly chinook salmon embryo cells (CHSE) and its derivatives. CHSE-sp cells, which cells completely adapted in suspension-stirred culture and the original of the aim, were selected and developed, and sublines of CHSE-sp were established as CHSE-ss (SS) cells for static-suspension and CHSE-ns (NS) cells for non-serum culture. SS cells stationary incubated at 4゚C in hydrophobic surface flask offered single cell suspension without enzymatic digestion and monolayr cell sheet for bio-assay in hydophilic dish even after 1 month. NS cells secreted the cell adhesion and growth substances in the culture medium. Calf type I collagen, inhibited the cell adhesion of CHSE-sp, were permitted the adhesion and spreading of cells by heat denature treatment.

  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 村野賢博: "魚類培養細胞の改良型培養について" 日本水産学会春季大会講演要旨集. #443 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金子由記子: "コラーゲン基質におけるマスノスケ細胞CHSE-spの接着・伸展について" 日本水産学会春季大会講演要旨集. #444 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 五十嵐麦子: "連続継代が可能な無血清培養系魚類細胞の初めての樹立" 日本水産学会春季大会講演要旨集. #445 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko Y., M.Igarashi, M.Iwashita, K.Suzuki, H.Kojima, S.Kimura and M.Hasobe: "Effects of fish and calf collagens as culture substrate in the adhesion and spreading process of established fish cells" In Vitro Cell. Dev.Biol. 31. 178-182 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Araki N., M.Yamaguchi, H.Nakano, Y.Wada and M.Hasobe: "A fish cell line CHSE-sp exposed to long-term cold temperature retains viability and ability to support viral replication" In Vitro Cell. Dev.Biol. 30. 148-150 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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