GENETIC SCREENING FOR THE HEREDITARY BLEEEDING DISORDERS IN JAPANESE BLACK CATTLE

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 06454131
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: MIYAZAKI UNIVERSITY
• Principal Investigator: OGAWA Hiroyuki MIYAZAKI UNIV.VET.SURG.ASSOC.PROF., 農学部, 助教授 (30012016)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Ryozi MIYAZAKI UNIV.VET.PATHOL.ASSOC.PROF., 農学部, 助教授 (90150169) ; GOTO Yositaka MIYAZAKI UNIV.VET.MICROBIOL.ASSOC.PROF., 農学部, 助教授 (30142136) ; KASEDA Yuziro MIYAZAKI UNIV.VET.HOSPITAL PROF., 農学部, 教授 (70041019)
• Project Period (FY): 1994 – 1996
• Keywords: Bovine / Bleeding disorder / Platelet dysfunction / von Willebran factor / Factor XIII / Cenetic screening

Research Abstract

From our investigations on three hundred of bleeding Japanese black cattle since 1982, we have reported that the sporadic hemorrhagic disease is not a single disease, but it originates in three diseases of Chediak-Higashi syndroml, hemophilia like disease and factor XIII deficiency. In this study, we attempt to find the carrier detection method in these bleeding disorders.
1) Segregation analyzes and quntitative genetic analyzes showed that Chediak-Higashi syndrome (C-HS), hemophilia like disease and factor XIII deficiency in cattle were simple autosomal recessive traits.
2) Von Willebrand factor activity assay and multimer analysis showed that Hemophilia like disease was von Wliiebrand disease type III.
3) Screening method for carrier detectin was not established in C-HS and vWd.
4) In factor XIII deficiency, plasma factor XIII activity was decreased in carriers and A mutation in the gene for factor XIII subunit A was detected and linkage of this mutation with the defect was confirmed. Then, factor XIII carriers could be screeened out by PCR-RFLP method successfully. Ten percent of sires tested were found to be carriers.

Research Products

• [Publications] 杜杰憲ほか: "臍帯出血を主徴とする黒毛和種牛の出血性疾患" 日獣会誌. 49巻4号. 237-240 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tu.C-H et al.: "Inheritance of Chediak-Higashi Syndrome in Jpn.Black Cattle" J.Vet.Med.Sci.58(6). 501-504 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] TU C-H,IKEYA M,WADA T,GOTO M,NONAKA I,MIKAWA K,ITO T,UCHIDA K,YAMAGUCHI R,MURAKAMI T,KASEDA Y,OGAWA H,OTSUKA H.: "Bleeding Disorder in Japanese Black Cattle with Umbilical Cord Bleeding as a Major Clinical Sign." J.Jan.Vet.Med.Assoc.49(4). 237-240 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] TU C-H,TAKAHASHI Y,KASEDA Y,UCHIDA K,YAMAGUCHI R,SUZUKI K,OGAWA H,OTSUKA H.: "Inheritance of Chediak-Higashi Syndrome in Japanese Black Cattle." J.Vet.Med.Sci.58(6). 501-504 (1996)
  • Description: 「研究成果報告書概要(欧文)」より