1996 Fiscal Year Final Research Report Summary
Mechanisms of cell division with protein phosphorylation.
| Project/Area Number |
06454138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
IKEGAMI Susumu Hiroshima University, Dept.Applied Biochemistry, Professor, 生物生産学部, 教授 (80011980)
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| Co-Investigator(Kenkyū-buntansha) |
SHIODA Masaki Kumamoto University, Dept Biological Sciences, Professor, 理学部, 教授 (80134526)
HOSOYA Hiroshi Hiroshima University, Dept.Biological Sciences, Professor, 理学部, 教授 (90183102)
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| Project Period (FY) |
1994 – 1996
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| Keywords | Myosin / Phosphorylation / P1 / NAAP / cdc2 kinase / Cyclin B |
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| Research Abstract |
Hosoya and his colleagues discovered that phosphorylated myosin is present in the contractile ring. This is a very importand discovery for the understanding of the mechanism of cell division. They also discovered the enzyme that selectively phosphorylates the light chain of myosin.
Shioda and his colleagues discovered a novel protein, P1, which regulates the initiation of DNA replication. P1, which is inactive in its unphosphorylated form, becomes active by phosphorylation. The enzyme responsible for the phosphorylation is found to be a DNA-dependent kinase.
Ikegami and his colleagues discovered a novel nucleic-acid associated protein designated as NAAP which was isolated from starfish oocytes. Oligonucleotides derived from the partial amino acid sequences of NAAP were used to clone NAAPcDNA and the entire cDNA sequence was determined. The transcript encoding NAAP was present only in growing oocytes but not in full-grown oocytes, embryos and adult tissues. The NAAP protein localized in the germinal vesicle of the full-grown oocyte and dispersed diffusely after germinal vesicle breakdown at the onset of meiosis. During oocyte maturation, NAAP changed the moleclar form in a cell-cycle dependent manner. It was found that Ser 145 of NAAP,which was unphosphorylated at the germinal vesicle stage, was phosphorylated during the M phase of meiosis.
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Research Products
(8 results)
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[Publications] Ohta, S., Uno, M., Yoshimura, M., Hiraga, Y., and Ikegami, S.: "Rhopaloic acid A : A novel norsesterterpene from a marine sponge, Rhopaloeides sp., which inhibits gastrulation of starfish embryos." Tetrahedron Lett.37. 2265-2266 (1996)
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[Publications] Shimizu, T., Hozumi, K., Horiike, S., Nunomura, K., Ikegami, S,.Takao, T., and Shimonishi, Y.: "A covalently crosslinked histone." Nature. 380. 32 (1996)
Description
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