1995 Fiscal Year Final Research Report Summary
Expression of vesicle-associated proteins during the course of differentiation of growth cones into synaptic terminals in nerve regeneration (synaptophysin, synaptotagmin, protein kinase C,etc)
| Project/Area Number |
06454142
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
IDE Chizuka Kyoto Univ., Anatomy & Neurobiology, Professor, 医学研究科, 教授 (70010080)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIZOGUCHI Akira Kyoto Univ., Anatomy & Neurobiology, Associate Professor, 医学研究科, 助教授 (90181916)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Keywords | regenerating axon / growth cone / vesicle associated protein / signal transduction protein / synaptophysin / synaptotagmin / Rab3A / protein kinase C |
|---|
| Research Abstract |
Growth cones of regenerating axons contain numerous heterogenous vesicles which are postulated to be partly involved in the supply of membranous components to the surface axolemma needed for the growth cone extension. We examined the localization of four synaptic vesicle-associated proteins (Rab3A,synaptophysin, synapsin-1, and synaptotagmin), and protein kinase C subtypes (alpha, beta, gamma, delta, epsilon and zeta) in regenerating growth cones including axonal sprouts at nodes of Ranvier, and in growth cones of cultured neurons.
All these molecules were localized in the axonal sprouts which appeared shortly after injury at nodes of Ranvier and in growth cones of extending axons as well, and in neurite growth cones of cultured neurons.
These findings indicate that growth cone vesicles are involved in the supply of membranous components by fusion with the surface axolemma in the manner similar to the exocytosis at synapse, and that protein kinase C functions as a key molecule in intracellular signalling for the growth of axons.
|
