1995 Fiscal Year Final Research Report Summary
TEMPERATURE RECEPTIVITY IN DISSOCIATED HYPOTHALAMIC NEURONS AND CULTURED PC-12 CELLS
| Project/Area Number |
06454152
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | KYUTO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
KIYOHARA Toshikazu Kyoto institute of Technology Department of Applied Biology, Professor, 繊維学部, 教授 (50071874)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Seiji Kyoto Institute of Technology Department of Applied Biology, Research Associate, 繊維学部, 助手 (30243124)
NAKASHIMA Toshihiro Kyoto Institute of Technology Department of Applied Biology, Associate Professor, 繊維学部, 助教授 (30128136)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Temperature receptivity / Whole cell clamp / Cold sensitivity / c-fos / Cold acclimation |
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| Research Abstract |
1, Rat pheochromocytoma cells (PC-12) were treated with nerve growth factor for 5 days. The effects of cooling on membrane characteristics and ionic currents were investigated. All cells tested showed depolarization associated with increase in input membrane resistance by cooling. Cooling also increased voltage-dependent outward-rectifier and sodium inward currents. The results suggest that PC-12 cells have some characteristics of cold-sensitive neurons and have sodium and potassium channels which respond non-linearly to temperature change.
2, Changes in the immunoreactivity for c-fos in the rat brains were investigated during cold acclimation. The sites of c-fos immunoresponded cells were classified into three types on the basis of time course of the c-fos expression. First, c-fos immunoreactivity was seen in the animals exposed to the cool environment for 3 h and 24 h, but not found in the cold adapted (14 days). Second, c-fos positive cells were persistently found in the rats exposed to cold from 3 h to 14 days. Third, c-fos immunoreactivity were not observed in the animals exposed to the cooling for 3 h and 24 h, but it appeared in the cold adapted. These data reveal that changes in the sites of c-fos immunoreactive cells are correlated with the alternation of thermogenic mechanism in the rat brains with cold acclimation.
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