1995 Fiscal Year Final Research Report Summary
Molecular pharmacology of 36kDa microfibril associated glycoprotein (36kDa-MAGP)
| Project/Area Number |
06454158
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Kagawa Medical School |
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Principal Investigator |
KOBAYASHI Ryoji Kagawa Medical School, Faculty of Medicine, Professor, 医学部, 教授 (00020917)
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| Project Period (FY) |
1994 – 1995
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| Keywords | extracellular matrix protein / Ca^<2+> binding protein / Smith-Magenis syndrome / elasitn / EF hand protein / blood vessel / microfibril / inhibitor |
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| Research Abstract |
A new extracellular matrix protein of 36kDa has been purified from bovine and porcine aorta. The protein, 36kDa microfibril associated glycoprotein (36kDa-MAGP), has a fibrinogen-like domain and contained the sequence Arg-Gly-Asp in the N-terminal region, which is the site for the association with cell and extracellular matrix. Immunoelection microscopy specified its location to elastin-microfibrils.
Using Ca^<2+>-dependent affinity chromatography on an isoquinoline sulfonamide (CKA1303) coupled agarose, we obtained pure form of 36kDa-MAGP.This compound should serve as a useful tool for clarifying the physiological roles of 36kDa-MAGP.During the synthesis and selection of S-100 protein antagonists derived from cinnamic acid and anthranilic acid, we discovered that N-acetyl anthranilic acid and 0-(3', 4'-dimethoxy cinnamoyl) salicilic acid strongly bind to 36kDa-MAGP.
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[Publications] Itoh H,Kobayashi R,Wakui H,Komatsuda A,Ohtani H,H,Miura AB,Otaka M,Masamune O,Andoh H,Koyama K,Sato Y,Tashima Y: "Mammalian 60-kDa Stress Protein (Chaperonin Homolog)." J.Biol.Chem.270. 13429-13435 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Oyama Y,Shishibori T,Yamashita K,Naya T,Nakagiri S,Maeta H,Kobayashi R: "Two distinct anti-allergic drugs, amlexanox and cromolyn, bind to the same kinds of calcium binding proteins, except calmodulin, in bovine lung extract." Biochem.Biophys.Res.Commun.240. 341-347 (1997)
Description
「研究成果報告書概要(欧文)」より
