1994 Fiscal Year Annual Research Report

オステオポンチントランスジェニックマウスの分子病理学

Research Project

Project/Area Number	06454194
Research Institution	大分医科大学
Principal Investigator	樋口安典大分医科大学, 医学部, 助教授 (60040284)
Co-Investigator(Kenkyū-buntansha)	瀬戸口美保子大分医科大学, 医学部, 助手 (20236110)
Keywords	osteopontin / metallothionein / transgene / α1-antitripsin
Research Abstract	1.OPTMの作出:メタロチオネイン(MT)プロモーターをマウスのOP遺伝子の5′上流に連結した変異遺伝子を作成し、現在C57BL/6Nマウスのトランスジェニックマウス(TM)を作成し、C57BL/6NマウスにおけるMTプロモーターのTM(MTTM)をこれまで12系統作出した。これらの各系統のうち4系統で第2世代の出生が観察されたが、第3世代で妊性が見られないか胎生期あるいは新生仔の段階で全部淘汰されMTTMの維持に困難を極めた。第1および第2世代の3匹を屠殺したが、肉眼および光顕的に著変は見られなかった。そこで肝臓で強く発現するα1アンチトリプシン(α1AT)をプロモーターにし、OP部をOP cDNAを用いたTMの作成をF1マウスで試みた。これにより現在まで10系統のα1ATTMを作出することに成功した。現在これらのTMの繁殖を開始したところで、諸解析はこれからである。

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Matsuura,K.: "Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide" Journal of Experimental Medicine. 179. 1671-1676 (1994)
[Publications] Hijiya,N.: "Cloning and characterization of the human osteopontin gene and its promoter" Biochemical Journal. 303. 255-262 (1994)
[Publications] Ishida,T.: "Enhancement of murine serum amyloid A3 (SAA3) mRNA expression by glucocorticoids and its regulation by cytokines" Journal of Leukocyte Biology. 56. 797-806 (1994)
[Publications] Nasu,K.: "Expression of wild type and mutated rabbit osteopontin in Escherichia coli,and their effects on adhesion and migration of P388D1 cells" Biochemical Journal. 307. 257-265 (1995)
[Publications] Yamamoto,S.: "Structure of the osteopontin gene and promoter" Annals of New York Academy of Science. (in press).
[Publications] Yamamoto,S.: "Effect of recombinant osteopontin on adhesion and migration of P388D1 cells" Annals of New York Academy of Science. (in press).
[Publications] Miyazaki,Y.: "Osteopontin.Role in cell signalling and adhesion" Annals of New York Academy of Science. (in press).
[Publications] 樋口安典: "CD14とエンドトキシンショック" 臨床免疫. 27. 349-356 (1995)
[Publications] Yamamoto,S.: "Leukocyte Typing V White Cell Differentiation Antigens" Springer Verlag (in press), (1995)
[Publications] Yamamoto,S.: "Leukocyte Typing V White Cell Differentiation Antigens" Springer Verlag (in press), (1995)
[Publications] 樋口安典: "現代病理学大系" 中山書店(印刷中), (1995)

1994 Fiscal Year Annual Research Report

オステオポンチントランスジェニックマウスの分子病理学

Principal Investigator

樋口 安典 大分医科大学, 医学部, 助教授 (60040284)

Research Products

[Publications] Matsuura,K.: "Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide" Journal of Experimental Medicine. 179. 1671-1676 (1994)

[Publications] Hijiya,N.: "Cloning and characterization of the human osteopontin gene and its promoter" Biochemical Journal. 303. 255-262 (1994)

[Publications] Ishida,T.: "Enhancement of murine serum amyloid A3 (SAA3) mRNA expression by glucocorticoids and its regulation by cytokines" Journal of Leukocyte Biology. 56. 797-806 (1994)

[Publications] Nasu,K.: "Expression of wild type and mutated rabbit osteopontin in Escherichia coli,and their effects on adhesion and migration of P388D1 cells" Biochemical Journal. 307. 257-265 (1995)

[Publications] Yamamoto,S.: "Structure of the osteopontin gene and promoter" Annals of New York Academy of Science. (in press).

[Publications] Yamamoto,S.: "Effect of recombinant osteopontin on adhesion and migration of P388D1 cells" Annals of New York Academy of Science. (in press).

[Publications] Miyazaki,Y.: "Osteopontin.Role in cell signalling and adhesion" Annals of New York Academy of Science. (in press).

[Publications] 樋口安典: "CD14とエンドトキシンショック" 臨床免疫. 27. 349-356 (1995)

[Publications] Yamamoto,S.: "Leukocyte Typing V White Cell Differentiation Antigens" Springer Verlag (in press), (1995)

[Publications] Yamamoto,S.: "Leukocyte Typing V White Cell Differentiation Antigens" Springer Verlag (in press), (1995)

[Publications] 樋口安典: "現代病理学大系" 中山書店(印刷中), (1995)

樋口安典大分医科大学, 医学部, 助教授 (60040284)