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1995 Fiscal Year Final Research Report Summary

Molecular pathology of osteopontin transgenic mice

Research Project

Project/Area Number 06454194
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionOita Medical University

Principal Investigator

HIGUCHI Yasunori  Oita Medical Univ.Pathology, assist.professor, 医学部, 助教授 (60040284)

Co-Investigator(Kenkyū-buntansha) SETOGUCHI Mihoko  Oita Medical Univ.Pathology, assistant, 医学部, 助手 (20236110)
Project Period (FY) 1993 – 1995
KeywordsTransgene / Osteopotin / Metallothionein / Cytomegalovirus / U2alpha globulin / alpha1-antitrypsin
Research Abstract

1.Establishment of transgenic mice (TM) with metallothionein (MT) promoter-driven mouse osteopontin (OP) gene and their analysis : A MT promotor-OP fusiongene was constructed, and everal founder mice were produced. However, use of the TM was impossible because of few number of litter mates and killing of new born TM by mother. Speciemens and samples from a few founder mice were subjected to biochemical and immunohistochemical analysis. Expression of transgenic RNA was abundant in the liver in TM on the basal diet. After maintenance with water containing ZnSO4 (50mM) for 4 days, expression of transgenic RNA was strong in the small intestine. Immunohistochemical analysis demonstrated OP expression in these organs in TM.Levels of OP in sera from TM after maintenance with water containing ZnSO4 (50mM) for 4 days were significantly higher than these animals maintained with normal water and nontransgenic mice. Founder mice were healthy and one of them is alive 2 years after birth. Autopsy ex … More amination of TM revealed no abnormal organs. 2.Establishment of trnansgenic mice (TM) with cytomegalovirus (CV) or U2alpha globulin (U2alpha) promoter-driven OP gene and their analysis : CV or U2alpha promoter-mouse osteopontin (OP) fusiongene was constructed, and a few founder mice were produced. However, we were anable to expand these TM.Speciemens and samples from a few founder mice were subjected to biochemical and immunohistochemical analysis. Northern blot and PAP analysis demonstrated OP in kidney in CVTM and in the liver in U2alpha TM.Levels of OP in sera from TM were significantly higher than nontransgenic mice. 3.Establishment of TM with alpha1-antitrypsin (alpha1AT) promoter-driven OP gene and their analysis : A alpha1AT-OP fusiongene was constructed, and several founder mice were produced in C57BL/6N/DBA/2F1 mice. Expansion of alpha1AT TM was easy. Backcross of heterozygote mouse to C57BL/6N to get TM of this backgrownd is now underway, reaching 6N.Northern blot and PAP analysis demonstrated OP in the liver in alpha1AT TM.Levels of OP in sera from TM were significantly higher than nontransgenic mice. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yamamoto,S.: "Structure of the osteopontin gene and promoter" Annals of New York Academy of Science. 760. 44-60 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,S.: "Effect of recombinant osteopontin on adhesion and migration of P388D1 cells" Annals of New York Academy of Science. 760. 378-382 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazaki, Y.: "Osteopontin : Role in cell signalling and adhesion" Annals of New York Academy of Science. 760. 334-342 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nasu,K.: "Expression of wild type and mutated rabbit osteopontin in Escherichia coli, and their effects on adhesion and..." Biochemical Journal. 116. 1082-1087 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口安典: "CD14とエンドトキシンショック" 臨床免疫. 27. 349-356 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口安典: "LPS結合蛋白の多様性" 臨床免疫. 27. 1510-1514 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishimura,H.: "The roles of γ δT cells in priming macrophages toproduce tumor necrosis factor-α" Eur. J. Immunol.25. 1465-1468 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Delude,R.L.: "CD14 enhances cellular responses to endotoxin without imparting ligand-specific recognition" Proc. Natl. Acad. Sci. USA. 92. 9288-9292 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口安典: "CD14とエンドトキシンショック" 臨床免疫. 27. 349-356 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口安典: "LPS結合蛋白の多様性" 臨床免疫. 27. 1510-1514 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本俊輔: "マクロファージ細胞表面上の分化抗原とその意義" 臨床免疫. 28. 37-48 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Hisaka et al.: "Establishment of transgenic mice expressing mouse osteopontin and their analysis" Journal of Experimental Medicine. (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,S.: "Leukocyte Typing V White Cell Differentiation Antigens" Springer Verlag, 788-790 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,S.: "Leukocyte Typing V White Cell Differentiation Antigens" Springer Verlag, 1091-1093 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口安典: "現代病理学大系" 中山書店, 95-110 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Delude,R.L.: "Bacterial Endotoxins Lopopolysaccharides from Genes to Therapy" Weiley-Liss, 487-497 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 1.Yamamoto, S., Hijiya, N., Miyazaki, Y., Setoguchi, M., Matsuura, K., Higuchi, Y., Akizuki, S: "Structure of the osteopontin gene and promoter" Annals New York Academy of Science. 760. 44-60 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 2.Yamamoto, S., Nasu, K., Ishida, T., Setoguchi, M., Higuchi, Y., Hijiya, N., Akizuki, S.: "Effect of recombinant osteopontin on adhesion and migration of P388D1 cells" Annals New York Academy of Science. 760. 378-382 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 3.Miyazaki, Y., Tashiro, T., Nagai, H., Setoguchi, M., Higuchi, Y., Yamamoto, S., Vassalli, P.: "Osteopontin : Role in cell signalling and adhesion" Annals New York Acadof Science. 760. 334-342 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 4.Nasu, K., Ishida, T., Setoguchi, M., Higuchi, Y., Akizuki, S.and Yamamoto, S.: "Expression of wild type and mutated rabbit osteopontin in Escherichia coli, and their effects on adhesion and migration of P388D1 cells." Biochemical Journal. 116 (5). 1082-1087

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 1.Yamamoto, S., Matsuura, K., Tamura, Y., Ishida, T., Higuchi, Y: Characterization and use of monoclonal antibodies against mouse CD14 in the Leukocyte Typing V White Cell Differentiation Antigens (ed by S.Schlossman). 788-790 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 2.Yamamoto, S., Yoshiyama, K., Nasu, K., Kataoka, M., Setoguchi, M., Higuchi, Y.: Human MS2 antigen Molecular cloning, primary amino acid sequence and tissue distribution in the Leukocyte Typing V White Cell Differentiation Antigens (ed by S.Schlossman). 1091-1093 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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