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1995 Fiscal Year Final Research Report Summary

Studies on purification isolation and immunological characterization of 47kD and 23kD polypeptide from blood stage P.falciparum parasite

Research Project

Project/Area Number 06454197
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionGunma University School of Medicine

Principal Investigator

SUZUKI Mamoru  School of Medicine, Gunma University, Professor, 医学部, 教授 (60056033)

Co-Investigator(Kenkyū-buntansha) NARIUCHI Hideo  School of Medicine, Gunma Univeraity, Lecture, 医科学研究所, 教授 (10012741)
NAKAMURA Masatoshi  School of Medicine, Gunma Univeraity, Lecture, 医学部, 助手 (10251092)
KANO Shigeyuki  School of Medicine, Gunma Univeraity, Lecture, 医学部, 講師 (60233912)
Project Period (FY) 1994 – 1995
Keywordsmalaria / vaccine / Western blot / polypeptide / electrophoresis / monoclonal antibody / affinity chromatography / protective immunity
Research Abstract

We have continued this research for several years and have reached the final stage of the study in 1995. The eventual aim of this study is the preparation of 47kD polypeptide which is expressed on the membrane of Plasmodium falciparum parasite at schizont stage of the development. The molecule was focussed in the study on immunoblotting using sera from patients manifesting acute stage clinical symptoms. It looks that the molecule works in the immune response evoked to overcome the acute fulminating stage of the disease. Started from this finding, preparation of the 47kD polypeptide was attempted by means of genetic engineering using cDNA or gDNA libraries. However.successful results were not obtained. In the present study, a preparative SDS-PAGE system was applied to the purification of the target molecule. A large amount of cultured P.falciparum parasites was solubilized and the prepared material was subjected to the purification with the use of Prep-cell econo-auomation system(Bio Rad)by continuous-elution electophoresis. The electrophoresed material was devidedP into 40 fractions each with 8.5ml elutate. A 15mu1 sampled specimen from each fraction was taken and the moleular weight was respectively measured using thin layred polyacril amide gel electrophoresis kit (Excel Gel SDS gradiant 8-18, Pharmacia Biotech). Fractions No.28-32 which contained the polypeptide of target molecular weight were pooled. A new type immuno-affinity chromatography (Affi-Gel Hz lmmunoaffinity) was coupled with anti-47kD monolonal antibody and the pooled material was applied to the prepared column. After running with a large amount of washing buffer, the molecule which was bound to the gel by the monoclonal antibody was eluted by a elution buffer. The resulted material is lyophilized and waits for the study of in vitro immune cell response.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Shigeyuki Kano: "Recrudescence of falcparum malaria following treatment with halofantrine" Journal of the Japanese Association for Infectious Diseases. 68. 536-538 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Donald J. Krogstad: "Drug discovery, development and deplyment: a report from the 28th joint conference of the U. S. -Japan parasitic diseases panels, Baltimore, Maryland, July 1993" American Journal of Troplcal Madicine & Hygiene. 51. 384-388 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akanmori B. D.: "Immunoglobulin G_2. isotype may have a protective role in Plasmodium berghei NK65 infection in immunised mice" Parasitology Research. 80. 638-641 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minato Nakazawa: "Differntial malaria prevalence among villages of the Gidra in lowland Papua New Guinea" Ttopical and Geographical Medicine. 46. 350-354 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeyuki Kano: "Localization of 47kD antigenic polypeptides in malaria parasite by confocal laser scanning miroscopy" Bioimages. 3. 13-17 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Agneza Safranj: "Functional polymeric microspheres for the detection of anti-NANP antibodies in falciparum malaria patients" Japanese Journal of Parasitology. 44. 170-173 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Agneza Safranj: "Functional polymeric microspheres synthesized by radiation polymerization" Radiation Physics and Chemistry 46 203〜206, 1995. 46. 203-206 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoru Kawai: "Rosette formation by Plasmodium coatneyi-infected erythrocytes of the Japanese Macaque (Macaca fuscata)" American Journal of Tropical Medicine and Hygiene. 53. 295-299 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Waki: "Interferon-gamma and the induction of protective IgG2a antibodies in non-lethal Plasmodium berghei infections of mice" Parasite Immunology. 17. 503-508 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeyuki Kano: "Prolongation of the QT interval observed in a Japanese patient with vivax malaria following treatment with halfantrine" The Journal of Japanese Association for infectious diseases. 69. 1408-1412 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akanmori B. D.: "Mousc rIL6 boosis spccific scrum anti-plasmodial Ig isotypes and suppresses parasitacmia in Plasmodium chaboudi chabaudi inlcction." Parasite Immunology. (accpted for publication). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawai S: "Kawai S, Kano S, Chen C, Suzuki M: The ettects of pyronaridine on the morphology of Plasmodium falciparum in Aotus Trivirgatus." Am J Trop Med Hyg,. (accepted for publication). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeyuki Kano, Hohta Masuda, Mamoru, Suzuki: "Recrudescence of falcparum malaria following treatment with halofantrine" Journal of the Japanese Association for Infectious Diseases. vol.68. 536-538 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Donald J.Krogstad, Mamoru Suzuki, Carole A.Long, Yoshiki Aoki, Akira Ishii, and Stephanie L.James: "Drug discovery, development and deployment : a report from the 28th joint conference of the U.S.-Japan parasitic diseases panels, Baltimore, Maryland, July 1993" American Journal of Tropical Medicine & Hygiene. vol.51. 384-388 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] B.D.Akanmori, S.Waki, M.Suzuki: "Immunoglobulin G_2, isotype may have a protective role in Plasmodium berghei NK65 infection in immunised mice" Parasitology Research. vol.80. 638-641 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minato Nakazawa, Ryutaro Ohtsuka, Koshio Kawabe, Tetsuo Hongo, Tsuguyoshi Suzuki, Tsukasa Inaoka, Tomoya Akimichi, Shigeyuki Kano, Mamoru Suzuki: "Differntial malaria prevalence among villages of the Gidra in lowland Papua New Guinea" Tropical and Geographical Medicine. 46 (6). 350-354 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kano, N.Nakamura, T.Murakami, H.Ishikawa, M.Suzuki: "Localization of 47kD antigenic polypeptides in malaria parasite by confocal laser scanning microscopy" Bioimages. vol.3. 13-17 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agneza Safranj, Shigeyuki Kano, Masaru Yoshida, Ryoichi Katakai, Mamoru Suzuki: "Functional polymeric microspheres for detection of anti-NANP antibodies in falciparum malaria patients" Japanese Journal of Parasitology. 44. 170-173 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agneza Safranj, Shigeyuki Kano, Masaru Yoshida, Hideki Omichi, Ryoichi Katakai Mamoru Suzuki: "Functional polymeric microspheres synthesized by radiation polymerization" Radiation Physics and Chemistry. 46. 203-206 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoru Kawai, Shigeyuki Kano, Mamoru Suzuki: "Rosette formation by Plasmodium coatneyi-infected erythrocytes of the Japanese Macaque (Macaca fuscata)" American Journal of Tropical Medicine and Hygiene. 53 (3). 295-299 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiji Waki, S.Uehara, K.Kanbe, H.Nariuchi and M.Suzuki: "Interferon-gamma and the induction of protective lgG2a antibodies in non-lethal Plasmodium berghei infections of mice" Parasite Immunology. 17. 503-508 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeyuki Kano, Akihiro Hayashi, Tsugiyasu Kaneda, Mamoru Suzuki: "Prolongation of the QT interval observed in a Japanese patient with vivax malaria following treatment with halfantrine" The Journal of Japanese Association for infectious diseases. 69. 1408-1412 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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