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1995 Fiscal Year Final Research Report Summary

Role of HSP65 in host protection against intracellular protozoan infection.

Research Project

Project/Area Number 06454200
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionTokushima University

Principal Investigator

HIMENO Kunisuke  University of Tokushima, professor School of Medicine, 医学部, 教授 (50112339)

Co-Investigator(Kenkyū-buntansha) SAKAI Tohru  University of Tokushima, assistant School of Medicine, 医学部, 助手 (40274196)
HISAEDA Hajime  University of Tokushima, assistant School of Medicine, 医学部, 助手 (50243689)
Project Period (FY) 1994 – 1995
KeywordsHeat shock protein / Protozoan infection / Toxoplasma / Leishmania / Virulence / gammadelta T cell / Protective immunity
Research Abstract

We previously reported that induction of host 65 kDa heat shock protein (HSP65) closely correlates with protection against Toxoplasma gondii (T.gondii) infection, and T cells, especially gammadelta T cells, play an important role in protection and HSP65 expression.
T.gondii infection induces HSP65 expression in peritoneal macrophages and depletion of gammadelta T cells resulted in marked attenuation of its espression. This T cell subset secretes IFN-gamma and TNF-alpha as determined by using RT-PCR method. These cytokines are known to activate macrophages. Neutralization of these cytokines with corresponding antibodies prior to infection suppresses HSP65 expression, indicating that HSP65 expression attributed by gammadelta T cells is dependent on these cytokines. We speculate that HSP65 participates in protection by means of preservation of macrophages, in coincident with its native role as "molecular chaperon". Peritoneal macrophages and macrophage cell line, J774 cells infected in vitro with T.gondii undergo apoptosis. Expression of HSP65 on these cells, being induced with above cytokines, prevents them from apoptosis. However, this effect is reversed by the addition of antisense oligonucleotide for this protein. These results indicate that HSP65 prevent apoptosis of macrophages, which contributes to protection.
A similar relationship between protective immunity and HSP65 expression was also seen in mice infected with Leishmania major and Trypanosoma cruzi.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hara,T.: "Human Vδ2^+γδ T cell tolerance to foreign antigen of Toxoplasma gondii." Proc.Natl.Acad.Sci.USA. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasutomo,K.: "The novel mechanism of Fas system-mediated apoptosis in mesangial cell : Implication to mesangial proliferative glomerulonephritis." Biochem.Biophys.Res.Commun.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chai,J-G,: "Dissociation between the mitogenic effect and antitumor activity of seed extract from Aeginetia Indica L." Immunopharmacology. 30. 209-215 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,A.: "Establishment of a xenogeneic chimera without GVHD in NK cell-depleted SCID mice by grafting rat fetal liver cells." Cell.Immunol.164. 176-181 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisaeda,H.: "γ/δ T cells play an important role in hsp65 expression and in acquiring protective immune responses against infection with Toxoplasma gondii." J.Immunol.155. 244-251 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai,T.: "Low level of immunoglobulin G2a subclass correlates with a deficiency in T helper cell function in LEC mutant rats." J.Vet.Med.Sci.57. 527-529 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久枝一: "G.I.Research (ストレス蛋白質と免疫応答)" 先端医学社, 8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 長澤秀行: "生体の科学(ストレス蛋白質と原虫感染防御)" 科学評論社, 4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara, T.: "Human Vdelta2^+ gammadelta T cell tolerance to foreign antigen of Toxoplasma gondii." Proc.Natl.Acad.Sci.USA. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasutomo, K.: "The novel mechanism of Fas systemmediated apoptosis in mesangial cell : Implication to mesangial proliferative glomerulonephritis." Biochem.Biophys.Res.Commun.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chai, J-G.: "Dissociation between the mitogenic effect and antitumor activity of seed extract from Aeginetia Indica L." Immunopharmacology. 30. 209-215 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa, A.: "Establishment of a xenogeneic chimera without GVHD in NK cell-depleted SCID mice by grafting rat fetal liver cells." Cell.Immunol.164. 176-181 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisaeda, H.: "gammadelta T cells play an important role in hsp65 expression and in acquiring protective immune responses against infection with Toxoplasma gondii." J.Immunol.155. 244-251 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai, T.: "Low level of immunoglobulin G2a subclass correlates with a deficiency in T helper cell function in LEC mutant rats." J.Vet.Med.Sci.57. 527-529 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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