1995 Fiscal Year Final Research Report Summary
In vivo analysis of molecular mechanism of lymphocyte migration in Peyer's patch
| Project/Area Number |
06454265
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University, School of Medicine |
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Principal Investigator |
MIURA Soichiro Keio University, Dept.of Medicine, Associate Professor, 医学部, 助教授 (50138012)
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| Co-Investigator(Kenkyū-buntansha) |
TSUZUKI Yoshikazu Keio University, Department of Medicine, 医学部・内科, 助手 (10236929)
SERIZAWA Hiroshi Kitasato Institute Hospital, Department of Medicine, 付属病院・内科, 医員 (60187870)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Peyer's patch / lymphocyte migration / postcapillary venule / intestinal lymphatics / long-chain fatty acid / lymphocyte subpopulation / alpha4-integrin / VIP (vasoactive intestinal peptide) |
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| Research Abstract |
Gut associated lymphoid tissue (GALT) plays an important role for intestinal mucosal defense against dietary and bacterial antigens. In this study, we have established an observatory system of lymphocyte migration in rat Peyer's patches using intravital fluorescence microscopy. Fluorescence labeled lymphocytes adhered to the postcapillary venules (PCV) of Peyer's patches after transient rolling along venular walls. Lymphocytes sticking to the venules increased in number at 10-20 minutes, with preferential adherence of CD4+ cells to venules of 25-50 mum and adherence of B cells to the venules of a wider size range. In this sticking process, adhesion molecules, alpha4-intgerin, L-selectin and CD18/ICAM-1 are shown to be involved. Especially we demonstrated that alpha4-integrin molecule plays a critical role in the early stages of T lymphocyte migration in Peyer's patches. After 30 min, lymphocytes started to extravasate from PCV moved into the interstitium. B cells migrated from venules more quickly than CD4+ cells. Subsequently T lymphocytes preferentially appeared in parafollicular microlymphatics, while few B cells were transported to lymphatics. We also found that alpha4-integrin plays a significant role in the process of transendothelial migration of T lymphocytes in PCV of Peyer's patches. Fat absorption significantly enhanced the rolling and sticking of T cells and subsequent migration into microlymphatics. On the other hand, vasoactive intestinal peptide (VIP) significantly inhibited the transendothelial migration and lymphatic transport without affecting lymphocyte adherence to PCV.By this study, we were able to demonstrate the sequential migration process of lymphocye subpopulations and obtained informations about their regulatory mechanisms.
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Research Products
(20 results)
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[Publications] Serizawa, H., Miura, S., Tashiro, H., Imaeda, H., Shiozaki, H., Ohkubo, N., Kimura, H., Tanaka, S.and Tsuchiya, M.: "Alteration of mucosal immunity after long-term ingestion of an elemental diet in rats." J.Parenteral Enteral Nutr. (JPEN). 18. 141-147 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ohkubo, N., Miura, S., Serizawa, H., Han, J.Y., Kimura, H., Imaeda, H., Tashiro, H.and Tsuchiya, M.: "In vivo effect of chronic administration of vasoactive intestinal peptide on gut-associated lymphoid tissues in rats." Regul.Pept.50. 127-135 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Nagata, H., Miyairi, M., Sekizuka, E., Morishita, T., Tatemichi, M., Miura, S.and Tsuchiya, M.: "In vivo visualization of lymphatic microvessels and lymphocytes migration in rat Peyer's patches." Gastroenterology. 106. 1548-1553 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miura, S., Imaeda, H., Ohkubo, N., Kimura, H., Han, J-Y., Tsuzuki, Y., Serizawa, H., Toda, K.and Tsuchiya, M.: "Effect of fatty acid absorption on lymphatic transport and intestinal secretion of immunoglobulins." Lymphology. 27(Suppl). 857-860 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, S., Miura, S., Tsuzuki, Y., Kimura, H., Ohkubo, N., Imaeda, H., Tashiro, H., Serizawa, H.and Tsuchiya, M.: "Elemental diet reduces progression of chronic inflammatory lesions in a rat model of granulomatous enteritis." In : Current Topics in Mucosal Immunology 1993, eds.by Tsuchiya, M., Yodoi, J., Hibi, T,Miura, S.Excerpta Medica(Amsterdam). 215-218 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Serizawa, H., Miura, S., Imaeda, H., Ohkubo, N., Kimura, H., Tashiro, H., Tsuzuki, Y.and Tsuchiya, M.: "Effect of continuous infusion of VIP (vasoactive intestinal peptide) on lymphocyte migration into GALT." In : Current Topics in Mucosal Immunology 1993, eds.by Tsuchiya, M., Yodoi, J., Hibi, T,Miura, S.Excerpta Medica (Amsterdam). 275-278 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Miura, S., Tsuzuki, T., Fukumura, D., Serizawa, H., Kurose, I., Imaeda, H., Kimura, H., Nagata, H., Tsuchiya, M.and Ishii, H.: "Intravital demonstration of sequential migration process of lymphocyte subpopulations in rat Peyer's patches." Gastroenterology. 109. 1113-1123 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miura, S., Serizawa, H., Tashiro, H., Imaeda, H., Shiozaki, H., Ohkubo, N., Tanaka, S.and Tsuchiya, M.: "The effect of an elemental diet on gut-associated lymphoid tisuue (GALT) in rats." IN : Advances in Mucosal Immunology, eds.by Mestecky J.et al.Plenum Press (New York, U.S.A.). 525-527 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, S., Miura, S., Suzuki, H., Ohkubo, N., Imaeda, H., Shiozaki, H., Tashiro, H., Serizawa, H., and Tsuchiya, M.: "Immunohistochemical study of rat Peyer's patches in peptidoglycan-polysaccharide complex-induced chronic enteritis." IN : Advances in Mucosal Immunology, eds.by Mestecky J.et al.Plenum Press (New York, U.S.A.). 1395-1398 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Serizawa, H., Miura, S., Tsuzuki, Y., Hamada, Y., Shigematsu, T., Hokari, R., Sekizuka, E., Tsuchiya, M.and H.Ishii: "Effect of neuropeptides on T cell migration in GALT (gut-associated lymphoid tissue)." Lymphology. 29(Suppl.1). 168-171 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Tsuzuki, Y., Miura, S., Suematsu, M., Kurose, I., Shigematsu, T., Kimura, H., Higuchi, H., Serizawa, H., Yagita, H., Okumura, K., and Ishii H.: "alpha4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer's patches." Int.Immunol.8. 287-295 (1996)
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「研究成果報告書概要(欧文)」より
