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1996 Fiscal Year Final Research Report Summary

Study of cells and peptides controling the hepatic sinusoidal blood flow

Research Project

Project/Area Number 06454269
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKurume University

Principal Investigator

TANIKAWA Kyuichi  Kurume University School of Medicine, 2nd Department of Medicine, Prof., 医学部, 教授 (10080649)

Co-Investigator(Kenkyū-buntansha) SAKAMOTO Masaharu  Kurume University School of Medicine, 2nd Department of Medicine, Assistant, 医学部, 助手 (60248367)
GONDO Kazuhisa  Kurume University School of Medicine, 2nd Department of Medicine, Assistant, 医学部, 助手 (00186909)
TORIMURA Takuji  Kurume University School of Medicine, 2nd Department of Medicine, Assistant, 医学部, 助手 (60197986)
UENO Takato  Kurume University School of Medicine, 2nd Department of Medicine, Assistant Prof, 医学部, 講師 (70176618)
Project Period (FY) 1994 – 1996
Keywordsliver cirrhosis / hepatic sinusoidal microcirculation / hepatic stellate cell (Ito cell) / endothelin-1 / endothelin-1 receptor / alpha-smooth muscle actin / nitric oxide / 神経終末
Research Abstract

We performed the following studies to understand the changes of hepatic sinusoidal microcirculation in liver cirrhosis.
1. Studies of human normal liver tissues and cirrhotic liver tissues.
Immunolocalization of alpha-smooth muscle actin (alpha-SMA) and endothelin-1 receptor (ET-1R) was significantly increased along the sinusoidal walls in cirrhotic liver compared with normal liver. These products were mainly localized in hepatic stellate cells (HSCs). However, nerve endings and immunolocalization of S-100 protein were markedly decreased in cirrhotic liver compared with normal liver.
2. Studies of hepatic microcirculation using a thioacetamide treated rat cirrhosis model.
The elevation of the portal pressure and the decrease of the portal and hepatic blood flows were induced by the injection of L-NMMA,which is a nitric oxide (NO) inhibitor, in the portal vein.
3. In vitro studies using rat cultured HSCs.
(1) Cultured rat HSCs contracted in response to the addition of ET-1, and relaxd in response to the elevation of intracellular NO with interluikin-1 beta treatment. Moreover, immunofluorescent intensity of anti-cyclic-GMP in HSCs was significantly increased after IL-1 beta addition compared with before IL-1 beta treatment.
(2) Immunofluorescent intensity of anti-alpha-SMA and anti-ET-1R in HSCs obtained from normal liver and cirrhotic liver or rats was examined with a confocal laser-scanning microscope.
Immunofluorescent intensity of these antibodies was significantly increased in HSCs of the cirrhosis group compared with the normal group.
Based on these results, the contraction and relaxation of HSCs through ET-1 and NO rather than through intralobular innervation are suggested to be deeply involved in hepatic sinusoidal microcirculation in liver cirrhosis.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 谷川久一: "エンドセリン受容体の肝内分布-伊東細胞の収縮と類洞内血流調節" 医学のあゆみ. 168. 675-678 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷川久一: "伊東細胞における細胞骨格とその機能" 医学のあゆみ. 169. 727-731 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] 上野隆登: "伊東細胞の形態と機能" 細胞. 26. 90-94 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kyuichi Tanikawa: "Hepatic sinusoidal cells and sinusoidal circulation" Journal of Gastroenterology and Hepatology. 10. S8-S11 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] 上野隆登: "伊東細胞の形態と機能" 病理と臨床. 13. 324-330 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Masaharu Sakamoto: "Relaxing effect of interluekin-1 on rat-cultured Ito cells" Hepatology. 25(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takato Ueno: "Hepatic stellate cells and intralobular innervation in human liver cirrhosis" Human Pathology. 28(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takato Ueno: "Intralobular innervation and lipocyte contractility" Nutrition. 13(in press). (1997)

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      「研究成果報告書概要(和文)」より
  • [Publications] 権藤和久: "肝類洞壁細胞研究の進歩第6巻" 谷川久一, 217 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] 上野隆登: "肝類洞壁細胞研究の進歩第7巻" 谷川久一, 261 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Takato Ueno: "Liver innervation" Takashi Shimazu, 484 (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] Takato Ueno: "Cells of the Hepatic Sinusoid" E. Wisse,D. L. Knook,C. Balabaud (in press), (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] 谷川久一: "Annual Review消化器1997" 戸田剛太郎、税所宏光、寺野彰、幕内雅敏, 338 (1997)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhisa Gondo, Takato Ueno, Masaharu Sakamoto, Shotaro Sakisaka, Michio Sata, Kyuichi Tanikawa: "Endothelin-1 binding site in rat liver" Progress in Hepatic Sinusoidal Cell Studies. Vol.6. 104-106 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kyuichi Tanikawa: "Intrahepatic distribution of endotheling receptors-contraction of Ito's cell and control of sinusoidal blood flow" Journal of Clinical and Experimental Medicine. 168. 675-678 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kyuichi Tanikawa, Takato Ueno: "Cytoskeleton and its function in Ito cell" Journal of Clinical and Experimental Medicine. 169. 727-731 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Takato Ueno, Kyuichi Tanikawa: "Morphology and function of Ito cell (fat-storing cell)" The cell. 26. 90-94 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Takato Ueno: "Contraction mechanism of Ito cell" Progress in Hepatic Sinusoidal Cell Studies. Vol.7. 1-10 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyuichi Tanikawa: "Hepatic sinusoidal cells and sinusoidal circulation" Journal of Gastroenterology and Hepatology. 10. S8-S11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takato Ueno, Masaharu Sakamoto, Kazuhisa Gondo, Kyuichi Tanikawa: "Morphology and function of Ito cell" Pathology and Clincal Medicine. 13. 324-330 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takato Ueno, Ryuichiro Sakata, Takuji Torimura, Hiroshi Sugawara, Masaharu Sakamoto, Sadataka Inuzuka, Michio Sata, Kyuichi Tanikawa: "Intralobular innervation and hepatic stellate cell in human liver cirrhosis" Cells of the Hepatic Sinusoid. 6(in press). (1996)

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  • [Publications] Takato Ueno, Masaharu Sakamoto, Kyuichi Tanikawa: "Hepatic intrinsic innervation and contraction of Ito cells" Liver innervation. 1. 95-101 (1996)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kyuichi Tanikawa, Takato Ueno: "Ito cell" Annual Review (The Digestive organs). 147-152 (1997)

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  • [Publications] Masaharu Sakamoto, Takato Ueno, Takuji Torimura, Hiroshi Sugawara, Ryuichiro Sakata, Seishu Tamaki, Kodo Sujaku, Riko Tsuji, Motoaki Kin, Sadataka Inuzuka Michio Sata, Kyuichi Tanikawa: "Relaxing effect of interleukin-1 on rat-cultured Ito cells" Hepatology. 25 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takato Ueno, Ryuichiro Sakata, Takuji Torimura, Masaharu Sakamoto, Hiroshi Sugawara, Michio Sata, Kyuichi Tanikawa: "Hepatic stellate cells and intralobular innervation in human liver cirrhosis" Human Pathology. 28 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takato Ueno, Kyuihi Tanikawa: "Intralobular innervation and lipocyte contractility" Nutrition. 13 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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