• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Molecular physiological study of contractile element of failing heart

Research Project

Project/Area Number 06454285
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tokyo General

Principal Investigator

MOMOURA Shin-ichi  University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部(病), 助手 (10190985)

Co-Investigator(Kenkyū-buntansha) AOYAGI Teruhiko  University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部(病), 助手 (10251240)
TAKAHASHI Toshiuyki  University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部(病), 助手 (40236302)
Project Period (FY) 1994 – 1995
Keywordsin vitro motility asaay / laser optical trap / centrifuge microscope / cardiac myosin / regulatory protein
Research Abstract

1. We have shown that catlytic products of ATP,ADP and Pi, have an inhibitory action on actomyosin sliding in vitro, This result suggests that cardac myosin is more resistant to ischemia than skeletal one. I intact muscle, ATP regenerating system provides optimal condition for ATP hydrolysis by myosin.
2. In vitro sliding of thin filament reconstituted from actin and troponin-tropomyosin complex was regulated by calcium concentration. Calcium sensitivity was reduced under various conditions such as acidosis and hypothermia, but reversed by calcium sensitizer. This effect of calcium sensitizer seemed to be mediated by regulatory protein.
3. We compared the force generating ability of cardiac myosin isoforms (V1 and V3) using two different in vitro force measuring systems, centrifuge microscope and laser optical trap. The two isoforms had different kinetic properties, but had similar force generating ability.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yamashita H et al: "ADP inhibits the sliding velocity of fluorescent actin filaments on cardiac and skeletal myosins." Circ Res. 74. 1027-1033 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sata M et al.: "A new in vitro motility assay technique to evaluate calcium sensitivity of the cardiac contractile proteins." Pflugers Arch. 429. 443-445 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sata M et al.: "MCI-154increases Ca^<2+>sensitivity of reconstituted thin filament A study using a novel i vitro motility assay technique" Circ Res. 76. 626-633 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugiura S et al.: "Force-velocity relations of rat cardiac myosin isozymens sliding on algal cell actin cables in vitro." Biophys Biochim Acta. 1231. 69-75 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sata M et al: "Pimobendan directly sensitizes reconstituted thin filament to slide on cardiac myosin" Eur J Pharmacol. 290. 55-59 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sata M et al.: "Phosphocreatine shuttle provides optimal condition for mechanical interaction of cardiac actomyosin." Circulation. 93. 310-317 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamahita H,Sata M,Sugiura S,Momomura S,Serizawa T,Iizuka M: "ADP inhibits the sliding velocity of fluorescent actin filaments on cardiac and skeletl myosins." Circ Res. 74. 1027-1033 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sata M,Yamashita H,Sugiura S,Fujita H,momomura S,Seirzawa T: "A new in vitro motility assay technique to evaluate calcium sensitivity of the cardiac contractile proteins" Pflugers Arch. 429. 443-445 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sata M,Sugiura S,Yamashita H,Fujita H,Momomura S,Serizawa T: "MCI-154 increases Ca2+sensitivity of reconstituted thin filament A study using a novel in vitro motility assay technique" Circ Res. 76. 626-633 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugiura S,Yamashita H,Sata M,Momomura S,Serizawa T,Oiwa K,Chaen S,Shimmen T,Sugi H.: "Force-velocity relations of rat cardiac myosin isozymes sliding on algal cell actin cables in vitro." Biophys Biochim Acta. 1231. 69-75 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sata M,Sugiura S,Yamashita H,Aoyagi T,Momomura S,Serizawa T: "Pimobendan directly sensitizes reconstituted thin filament to slide on cardiac myosin" Eur J Pharmacol. 290. 55-59 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sata M,Sugiura S,Yamashita H,Fujita H,Momomura S,Serizawa T: "Phosphocreatine shuttle provides optimal condition for mechanical interaction of cardiac actomyosin." Circulation. 93. 310-317 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi