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1995 Fiscal Year Final Research Report Summary

Role of Nerve Growth Factor Receptor in Differentiation, Maturation and Spontaneous Regression of Human Neuroblastoma

Research Project

Project/Area Number 06454303
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionMiyazaki Medical College

Principal Investigator

SUGIMOTO Tohru  Miyazaki Medical College, Pediatrics, Professor, 医学部, 教授 (90117888)

Project Period (FY) 1994 – 1995
KeywordsNeuroblastoma / Differentiaion / Maturation / Nerve Growth Factor Receptor
Research Abstract

Nerve growth factor (NGF) plays an important role in the cell differentiton, maturation and survival of neural crest cells. The purpose of this study is to characterize the function of NGF,NGF receptor, signal transduction in cell differentiation, maturation and spontaneous regression of human neuroblastoma (NB), and eventually to improve the poor prognosis of patients with advanced NB.The following results were obtained.
1) trk A (high affinity receptor of NGF) expressions in NB cell lines :
The trk A expressions were observed in 6 of 14 NB cell lines (43%).
2) trk A and fos expressions by NGF treatment :
Alteration of trk A and fos (early gene) expressions by NGF treatment was determined in 3 NB cell lines, including one trk A negative expressing KP-N-RT cell line, and two trk positive expressing NB-1 and KP-N-SIFA cell lines. The expression of fos was not induced in KP-N-RT,whereas these were induced in NB-1 and KP-N-SIFA.
3) trk A and fos expressions by gamma-interferon or retinoid folowing by NGF treatment :
The expressions of trk A and fos were augmented by either gamma-interferon or retinoid folowing by NGF treatment, as compared with NGF treatment alone. Phosphorylation of trk A protein was observed by Western blot analysis. These resulsts revealed trk A positive expressing NB cell lines could respond to NGF,and cell signal could be transducted into fos early gene in nucleus. This transdution was augmented by gamma-interferon or retinoid treatment.
Thus the analysis of cell signal tranduction by NGF may distinguish cell differentiation, maturation and spontaneous regresion observed in infant NB,or cell proliferation observed in child NB.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] A.Shikata, T.Sugimoto, H.Hosoi, Y.Sotozono, T.Shikata, T.Sawada, LF, Parada: "Increased expression of trk proto-oncogene by γ-interferon in human neuroblastoma cell lines." Jpn. J. Cancer Res.85. 122-126 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Tanaka, M.Tanabe, T.Sugimoto, N.Ida, T.Sawada: "Age-related profile of neuroblastoma : A comparison of tumors detected by mass-screening with those detected clinically." Acta Paediatr. Jap.37. 154-159 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Tanaka, E.Hiyama, T.Sugimoto, T.Sawada, M.Tanage, N.Ida: "Trk gene expression in neuroblastoma : The clinical signeficance of an immunohistochemical study" Cancer. 76. 1086-1095 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉本 徹: "ヒト神経芽腫の臨床・細胞生物学的特性と予後" 日本小児科学会雑誌. 98. 1511-1514 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉本 徹、堀井 由博、高橋 克仁、峯 宏、澤田 淳: "神経芽腫の細胞分化と分化誘導療法" Biotherapy. 9. 862-872 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉本 徹、堀井 由博、澤田 淳: "III 臨床編、神経芽腫 癌細胞の分化誘導とアポトーシス(穂積本男編)" 共立出版, 20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Shikata, T.Sugimoto, H.Hosoi, Y.Sotozono, T.Shikata, T.Sawada, LF,Parada :"Increased expression of trk proto-oncogene by gamma-interferon in human neuroblastoma cell lines." Jpn.J.Cancer Res.85. 122-126 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Tanaka, M.Tanabe, T.Sugimoto, N.Ida, T.Sawada :"Age-related profile of neuroblastoma : A comparison of tumors detected by mass-screening with those detected clinically." Acta Paediatr.Jap.37. 154-159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Tanaka, E.Hiyama, T.Sugimoto, T.Sawada, M.Tanabe, N.Ida :"Trk gene expression in neuroblastoma : The clinical significance of an immnohistochemical study." Cancer. 76. 1086-1095 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Sawada, T.Shikata, T.Matsumura, H.Kawakatsu, T.Sugimoto and Members of the Neuroblastoma Screening Study Group :"Analysis of 598 cases of neuroblastoma detected by screening and changes in the age distribution and incidence of neuroblastoma patients after mass screening in infants in Japan." Advances in Neuroblastoma Res. (ed.A.E.Evans) Wiley-Liss. 371-375 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ida, S.Kobayashi, T.Taki, R.Hnada, F.Bessho, S.Yamamori, T.Sugimoto, Y.Hayashi :"EWS-FLI-1 and EWS-ERG chimeric mRNAs in Ewing's sarcoma and primitive neuroectodermal tumor." Int.J.Cancer. 63. 500-504 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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