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1995 Fiscal Year Final Research Report Summary

Cornified Cell Envelope Formation of Psoriatic Keratinocytes

Research Project

Project/Area Number 06454312
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionAsahikawa Medical College

Principal Investigator

IIZUKA Hajime  Asahikawa Medical College Department of Dermatology, Professor and Chairman, 医学部, 教授 (90113513)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hidetoshi  Asahikawa Medical College Deparment of Dermatology, Instructor, 医学部附属病院, 講師 (00216748)
YAMAMOTO Akemi (ISHIDA Ak)  Asahikawa Medical College Department of Dermatology, Instructor, 医学部附属病院, 講師 (30241441)
Project Period (FY) 1994 – 1995
Keywordspsoriasis / cornified cell envelope / involucrin / loricrin / turnover time / keratinization / AP-1 / 乾癬
Research Abstract

Epidermal keratinocytes form a cornified cell envelope beneath the plasma membrane during the late stages of differentiation. In psoriasis, the expression pattern of the precursor proteins is known to be deranged ; involucrin expression is increased and loricrin expression is decreased. Using light and electron microscopic immunohistochemistry, we compared normal and psoriatic cornified cell envelope formation. In normal epidermis.cornified cell envelopes were observed from the deepest cornified cells or when present, from the transitional cells, increasing in thickness and changing electron densities with maturation. In psoriatic epidermis, cornified cell envelope formation started earlier, one to several cells below the cornified layr. The normal cornified cell envelope was shown to be involucrin positive only at a very early stage, whereas psoriatic cornified cell envelope showed persistent involucrin immunoreactivity. The results suggest that in normal skin, involucrin is the major … More constituent of the cornified cell envelope only in its early stages of assembly. In contrast, cornified cell envelope formation seems to be initiated prematurely in psoriatic skin, were involucrin remains the major constituent of the cornified cell envelope during maturation.
Transcriptional enhancer factor 1 (TEF-1), which binds to SV40 enhancer, is a nuclear protein expressed in various cells including keratinocytes. TEF-1 protein was shown to be highly expressed on the basal cell layr. We analyzed involucrin promoter activity of the INV-CAT vector, which was constructed by connecting the 5' upstream region of involucrin gene to the CAT reporter gene. Co-transfection of TEF-1 expression vector with INV-CAT vector significantly the INV-CAT promoter activity. The repression was also observed by transfection of the GAL4-TEF-1 vector, which was constructed by replacement of the TEF-1 DNA binding domain by the GAL4 activator domain. This suggests that TEF-1-induced repression is due to interference/squelching of a limiting transcriptional intermediary factor that is essential for involucrin expression. TEF-1 dependent-repression of involucrin gene expression might explain the suprabasal involucrin expression in the epidermis.
The concept of epidermal architecture that depends on epidermal turnover time was established. The epidermal architecture is determined, not by a simple proliferative condition, but rather by an epidermal turnover time (that depends both on cell number as well as on the proliferative condition). This is in relation to 'keratinization process' that requires a definite time to be completed. Using the concept, hyperproliferative 'psoriasiform' epidermis and hypoproliferative 'atrophic' epidermis are naturally described. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Ishida-Yamamoto A-Iizuka. H: "Differences in involucrin Tmmunolabeling with in cornified cell envelopes in normal and psoriatic epidermls" J Invest Dermatol. 104. 391-395 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida-Yamamoto A. Iizuka H. et al: "Immunoelectorom microscopic analysis of cornified cell envelope formation of normal and psoriatic epidermis" J Histochem Cytochem. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi H. Iizuka H. et al: "Repression of involucrin gene expression by transcriptional enhancer factor (TEF)-1" Arch Dermatol Res. 287. 740-746 (1995)

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  • [Publications] Takahashi H Iizuka. H et al: British J Dermatol. (印刷中).

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      「研究成果報告書概要(和文)」より
  • [Publications] Iizuka H: "Epidermal architecture that depends on turn over time" J Dermatological Science. 10. 220-223 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Iizuka H. Ishida-Yamamot A, et al: "Epidermal remodelling in psoriasis" British J Dermatol. (印刷中).

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      「研究成果報告書概要(和文)」より
  • [Publications] Iizuka H: "Epidermal turnover time" J.Dermatol Sci. 8. 215-217 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tsutsui M,Tamura T,Takahashi H,Hashimoto Y,Iizuka H: "Stimulatory guanine nucleotide binding protein in pig epidermis : transient increase of the 45KDa cholera toxin substrate (Gs-alpha) in the tape stripping-induced hyperproliferative epidermis" Epi Cell Biol. 3. 161-167 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida-Yamamoto A,Iizuka H: "Differences in involucrin immunolabeling within cornified cell envelopes in normal and psoriatic epidermis" J.Invest Dermatol. 104. 391-395 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takagi A,Iizuka H: "UVB-induced calmodulin increase in pig epidermis : analysis of the effect of the calmodulin antagonist, W-13." Arch Dermatol Res. 287. 326-332 (1995)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto Y,Tsutsui M,Matsuo S,Iizuka H: "Flow cytometric analysis of pig epidermal keratinocytes : effects of ultraviolet B irradiation (UVB) and topical PUVA treatment" J Dermatol Sci. 10. 16-24 (1995)

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  • [Publications] Ishida-Yamamoto A,Iizuka H,Manabe M,O'Guin WM,Hohl D,Kartasova T,Kuroki T,Roop DR,Eady RAJ: "Altered distribution of keratinization markers in epidermolytic hyperkeratosis." Arch Dermatol Res. 287. 705-711 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi H,Kobayashi H,Matsuo S,Iizuka H: "Repression of involucrin gene expression by transcriptional enhancer factor (TEF) -1" Arch Dermatol Res. 287. 740-746 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto Y,Tsutsui M,Matsuo S,Iizuka S,Iizuka H: "Topical tacalcitol (1,24- (R) -dihydroxyvitamin D3) induces a transient increase in thymidine incorporation and calmodulin content in pig epidermis following tape stripping in vivo" J Dermatol Sci. 10. 196-202 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iizuka H: "Epidermal architecture that depends on turnover time" J Dermatol Sci. 10. 220-223 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi H,Kobayashi H,Hashimoto Y,Matsuo S,Iizuka H: "Interferon-gamma-dependent stimulation of Fas antigen in SV40-transformed human keratinocytes : modulation of the apoptoticprocess by protein kinase C" J Invest Dermatol. 105. 810-815 (1995)

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  • [Publications] Iizuka H,Takahashi H,Ishida-Yamamoto A,Hashimoto Y: "Lack of evidence for adenylate cyclase-modulation of normal pig epidermis by activators of epidermal phospholipase C" Epi Cell Biol. 4. 35-41 (1995)

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  • [Publications] Tamura T,Takahashi H,Iizuka H: "Protein kinase C-dependent modulation of stimulatory guanine nucleotide binding protein of fetal rat skin keratinocytes" Arch Dermatol Res. (in press).

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  • [Publications] Takahashi H,Tamura T,Iizuka H: "1,25-Dihydroxyvitamin D3 increased beta-adrenergic adenylate cyclase response of fetal rat keratinizing epidermal cells (FRSK cells)" J Dermatol Sci. 11. 121-128 (1996)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida-Yamamoto A,Eady RAJ,Watt FM,Roop DR,Hohl D,Iizuka H: "Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis" J Histochem Cytochem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi H,Kinouchi M,Tamura T,Iizuka H: "Decreased beta2-adrenergic receptor-, loricrin-, and increased involucrin-mRNA transcripts in psoriatic involved versus uninvolved epidermis : analyzes by reverse transcription-polymerase chain reaction" Br J Dermatol. (in press).

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      「研究成果報告書概要(欧文)」より
  • [Publications] Iizuka H,Ishida-Yamamoto A,Honda H: "Epidermal remodeling in psoriasis" Br J Dermatol. (in press).

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      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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