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1995 Fiscal Year Final Research Report Summary

MOLECULAR ANALYSIS OF STRUCTURE-FUNCTION RELATIONSHIP OF GLUCOSE TRANSPORTER AND INSULIN ACTION

Research Project

Project/Area Number 06454333
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionYAMAGUCHI UNIVERSITY

Principal Investigator

OKA Yoshitomo  YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (70175256)

Co-Investigator(Kenkyū-buntansha) ZAITU Yuzuru  YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE INSTRUCTOR, 医学部・附属病院, 医員
YOSHIDA Tuyoshi  YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE INSTRUCTOR, 医学部・附属病院, 医員
TANIZAWA Yukio  YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE ASSISTSNT PROFESSOR, 医学部・附属病院, 助手 (00217142)
Project Period (FY) 1994 – 1995
Keywordsglucose transporter / glucose transport / molecular engineering / fructose transport
Research Abstract

The biological and biochemical properties of glucose transpoter isoforms have been investigated. The rat GLUT5 cDNA was transfected into Chinese hamster ovary cells with an expression vector. GLUT5 was exclusively targeted to the plasma membrane and exhibited a transport activity, not for glucose, but for fructose with much higher affinity than GLUT2, the other glucose transporter with fructose transport activity. Interestingly, GLUT5 was not photolabeled with cytochalasin B,while a similar amount of GLUT1 was adequately photolabeled under the same experimental conditions. In addition, to elucidate the domains responsible for transport of glucose/fructose in GLUT1 and/or GLUT5, several chimeric GLUT1/GLUT5 proteins were expressed and their glucose transport and/or fructose transport activities were examined. The intracellular middle loop as well as the region encompassing from the 7th through the 12th membrane spanning domain were observed to have a crucial role in glucose transport of GLUT1, while replacement of the N-terminal half or the intracellular C-terminal region with the corresponding region of GLUT5 did not induce a marked effect on glucose transport activity. In contrast, both the N-terminal half from the N-terminus through the 6th membrane spanning domain and the intracellular C-terminal region were mandatory for fructose transport of GLUT5. Thus, GLUT5 is a transporter exclusively for fructose and the structure required for fructose transport is more stringent than that for glucose transport in hexose transporter proteins.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Ishihara H, et al.: "Inhibition of pancreatic β-cell glucokinase by antisense RNA expression in transgenic mice : mouse strain-dependent alteration of glucose tolerance." FEBS Letters. 371. 329-332 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tadokoro C, et al.: "Expresson and localization of glucose trandporter 1(GLUT1) in the rat oviduct ; a possible supplir of glucose to embryo during early embryonic development." Biochem Biophys Res Commun. 214. 1211-1218 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida K, et al.: "Regulation of glucose transporter I(GLUT1) gene expression by epidermal growth factor in bovine comeal endothelial cells." Japanese Journal Of Ophthalmology. 39. 225-232 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inukai K, et al.: "Characterization of rat GLUT5 and functional analysis of chimeric proteins of GLUT1 glucose transorter and GLUT5 fructose transporter." Endocrinology. 136. 4850-4857 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara H, et al.: "Human GLUT-2 overexpression does not affect glucose-stimulated insulin secretion in MIN6 cells." Am. J. Physiol.269. 897-902 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katagiri H, et al.: "A novel isoform of syntaxin-binding protein homologous to yeast Secl expressed ubiquitously in mammalian cells." J Biol Chem. 270. 4963-4966 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara H,et al.: "Overexpression of hexokinase I but not GLUT1 glucose transporter alters concentration-dependency of glucose-stimulated insulin secretion in pancreatic b-cell line MIN6." J.Biol Chem. 269. 3081-3087 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inukai K,et al.: "Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin B photolabelling of the GLUT1 glucose transporter." Biochem.J.302. 355-361 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita Y,et al.: "Glucose transporter GLUT1 mRNA expression in the ontogeny of glucose incorporation in mouse preimplantation embryos." Biochem Biophys Res Commun. 199. 1525-1531 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita Y,et al.: "Application of reverse transcription polymerase chain reaction to study the glucose transporter protein gene (GLUT) expression in preimplantation mouse embryo." J Mamm Ova Res. 11. 1-7 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oka Y.: "NIDDM-genetic marker ; glucose tarasporter, glucokinase, and mitochondria gene." Diabetes Reserch and Clinical Practice. 24. 117-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tadokoro C,et al.: "Expresson and localization of glucose trandporter 1 (GLUT1) in the rat oviduct ; a possible supplir of glucose to embryo during early embryonic development." Biochem Biophys Res Commun. 214. 1211-1218 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inukai K,et al.: "Characterization of rat GLUT5 and functional analysis of chimeric proteins of GLUT1 glucose transorter and GLUT5 fructose transporter." Endocrinology. 136. 4850-4857 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara H,et al.: "Human GLUT-2 overexpression dose not affect glucose-stimulated insulin secretion in MIN6 cells." Am.J.Physiol. 269. 897-902 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida K.et al.: "Regulation of glucose transporter 1 (GLUT1) gene experssion by epidermal growth factor in bovine corneal endothelial cells." Japanese Journal Of Ophthalmology. 39. 225-232 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katagiri H,et al.: "A novel isoform of syntaxin-binding protein homologous to yeast Secl expressed ubiquitously in mammalian cells." J Biol Chem. 270. 4963-4966 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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