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1995 Fiscal Year Final Research Report Summary

Role of apoptosis on human follicular growth and analyzes of regulattry mechanism of the apoptosis in granulosa cells

Research Project

Project/Area Number 06454471
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionNagoya University

Principal Investigator

SUGANUMA Nobuhiko  Nagoya Univ., School of Medicine, Associate Prof., 医学部, 助教授 (30179113)

Project Period (FY) 1994 – 1995
Keywordsgranulosa cell / rendometrial cell / apoptosis / TUNEL method / Fas-antigen / Bcl-2 / Le^y
Research Abstract

1) We analyzed apotosis by TUNEL method in the human granulosa cells obtained when in vitro fertilization was performed. The granulosa cells were cultured, and time-couse of apoptosis occurrence were analyzed with or without hCG in the culture media, however, some samples showed apoptosis and the others showed no apoptosis throughout the course. No effects of hCG were observed. These results indicated that the apoptosis in the granulosa or luteal cells might not involved the regulation of cell turn-over in these cells.
2) To clarify whether apoptosis is involved endometriosis, we obtained eutopic endometrial tissues along with endometriotic tissues from the adenomyosis in 12 cases and from the ovary in 12 other cases. Apoptosis-induced DNA fragmentation was detected by the TUNEL method, and immunostaining with a monoclonal antibody against the Fas, Le^y or bcl-2 was also performed using the same tissue section. Analysis showed that apoptosis was involved in all the ovarian endometriotic tissues but in only 2 of the 12 adenomyotic tissues and in 5 of the 24 eutopic endometrial tissues. None of these cases correlated to phases of the menstrual cycle. The expression of bcl-2 in the eutopic endometrial and adenomyotic tissues was limited to be proliferative phase, and was observed in only one of the 12 cases of ovarian endometriosis. Fas and Le^y were expressed randomly across a wide range in both the eutopic and ectopic endometrial tissues. These results suggest that features of ovarian endometriosis is defferent from adenomyosis and eutopic endometrium in terms of apoptosis involvement.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Ikuyo Kondo: "Embryo quality in faild in vitro fertilization patients." Assisted Reproduction Review. 4. 80-84 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiro Inagaki: "Development of an effective and simple insemination method for the in vitrofetilization of patients with male factor infertility." Asia-Oceania J.Obstec.Gynaecol.20. 375-382 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 近藤育代: "外因性ステロイドホルモンによりコントロールされた子宮内膜への凍結受精卵融解胚移殖の妊娠例" 日産婦誌. 46. 345-348 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤智子: "着床における自己抗体の意義-体外受精胚移殖における検討" 日本受精着床学会誌. 11. 69-72 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼信彦: "名古屋大学医学部附属病院分院" 臨床婦人科産科. 48. 170-171 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼信彦: "遺伝子組み換えの産婦人科領域への応用" 日産婦誌. 47. 1189-1193 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼信彦: "遺伝子組み換えの産婦人科への応用-その現況と将来-" 産婦人科治療. 71. 553-557 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼信彦: "アポトーシスからみた子宮内膜症" 産婦人科の実際. 45,(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo, I.Suganuma N: "Embryo quality in failed in vitro fertilization patients." Assisted Reproduction Review. 4. 80-84 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inagaki S,Suganuma N,Kitagawa T,Fukugaki H,Nakamura K,Kondo I,Tomoda Y: "Development of an effective and simple insemination method for the in vitro fertilization of the patients with male factor infertility." Asia-Oceania J Obstet Gynaecol. 20. 375-382 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo I,Nakamura K,Fukagaki H.Suganuma N.Hanai K,Furuhashi M,Tomoda Y: "Pregnancy after frozen-thawed embryo transfer to endometrium controlled by the replacemant of exogenous ovarian steroid hormones. (Japanese)" Acta Obstet Gynaec Jpn. 46. 345-348 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando T,Sun JR,Tsukahara S,Kitagawa T,Moriwaki T,Furui K,Kondo I,Suganuma N: "The role of autoantibodies in implantation of embryos. (Japanese)" Journal of Fertilization and Implantation [Tokyo]. 11. 69-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N: "Branch Hospital, Nagoya University School of Medicine. (Japanese)" Clinical Gynecology and Obstetrics. 48. 170-171 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N: "Application of molecular biological techniques for obstetric and gynecologic Field. (Japanese)" Acta Obstet Gynaec Jpn. 47. 1189-1193 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N: "Application of Molecular Biological Techniques for obstetrics and gynecology-present and future.(Japanese)" Obstetrical and Gynecological Therapy. 71. 553-557 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N: "Apoptosis in endometriotic tissues. (Jaoanese)" Obstetrical and Gynecological Practice. 45 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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