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1995 Fiscal Year Final Research Report Summary

A Biochemical Study on the Role of Amnion and Decidua Vera Tissues in the Maintenance of Human Pregnancy

Research Project

Project/Area Number 06454472
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SAGAWA Norimasa  Kyoto University, Graduate School of Medicine, Department of Gynecology and Obstetrics, Associate Professor, 医学研究科, 助教授 (00162321)

Co-Investigator(Kenkyū-buntansha) ITOH Hiroaki  Kyoto University, Graduate School of Medicine, Department of Gynecology and Obst, 医学研究科, 助手 (70263085)
KONISHI Ikuo  Kyoto University, Graduate School of Medicine, Department of Gynecology and Obst, 医学研究科, 講師 (90192062)
Project Period (FY) 1994 – 1995
Keywordsamnion / amniotic fluid / brain natriuretic peptide / decidua vera / endothelin / maintenance of pregnancy / phospholipse D / prostaglandin
Research Abstract

To elucidate the role of amnion and decidua tissues in the maintenance of human pregnancy, the production of endothelin (ET) and brain natriuretic peptide (BNP), and the expression of receptors for these peptides in intrauterine tissues were investigated. In addition, we examined the physiological role of phospholipase D (PLD) in amnion.
The ET production by cultured amnion cells was stimulated by various cytokines and growth factors present in amniotic fluid. Ligand binding analysis and Northern blot analysis indicated that both ET-A receptor and ET-B receptor are present in chorion laeve, decidua vera, and myometrium, but not in the amnion tissue at term. However, amnion tissue in the second trimester expressed ET-A receptor.
The BNP production by cultured amnion cells was dose-dependently stimulated by TGF-beta, but was inhibited by cortisol or EGF.The TGF-beta augmented BNP production was blocked by the simultaneous treatment with cortisol or EGF.The receptor for BNP (particulate gua … More nylate cyclase A-type : GC-A) was identified in chorion, decidua, and myometrium by both cGMP generation assay and Northern blot analysis. However, GC-A was not detected in amnion tissues in the second trimester and at term. Thus, BNP secreted from amnion cells may not act on amnion tissue, but on the decidua and myometrium, and generate cGMP,the second messenger of BNP,and finally block uterine contraction during the second trimester. Such an inhibition of uterine contraction by BNP may be attenuated at term when the BNP secretion from amnion cells are suppressed by cortisol and EGF.
We previously reported the presence and biochemical characteristics of PLD in amnion rissue. The PLD activity in cultured amnion cells has been shown to be regulated by protein kinase C and Ca^<2+>. Phosphatidic acid (PA), the primary product of PLD,stimulated the PGE_2 secretion from cultured amnion cells. Moreover, PLD enhanced the ET secretion and inhibited the BNP secretion from cultured amnion cells. Thus, PLD plays important roles in the regulation of function of amnion cells and maintenance of human pregnancy.
In conclusion, various bioactive substances in the amniotic fluid of fetal origin act on amniotic membrane, modulate the production of ET,BNP,PA or PGE_2, and finally regulate the uterine contractility, v.i.z.the maintenance of pregnancy. Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] H. Itoh, N. Sagawa, et al.: "Transforming growth factor-β stimulateds, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells." Journal of Clinical Endocrinology and Metabolism. 79(1). 176-182 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Itoh, N. Sagawa, et al.: "Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy." Biochemical and Biophysical Research Communications. 203(1). 602-607 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N. Sagawa, et al.: "Current topic: The role of amniotic endothelin in human pregnancy." Placenta. 15. 565-575 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N. Sagawa, et al.: "Secretion of endothelin by avascular amnion tissue: Possible roles in human pregnancy." Endocrine J.41(Suppl). S117-S125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumiko Inamori, Norimasa Sagawa, et al.: "Activation of phospholipase D in cultured human amnion cells." Reproduction, Fertility and Development. 7(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaki Hasegawa, Norimasa Sagawa, et al.: "Endothelin 1-like immunoreactivity and endothelin receptors in the human placenta from normotensive and hypertensive pregnancies." Journal of Perinatal Medicine. 23(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N. Sagawa, et al.: "Biochemical characterristics on phospholipase D in human amniotic membrane. In:Current Progress in Perinatal Medicine, (eds)E. V. Cosmi et al." The Parthenon Publishing Group, New York and London, 5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐川典正、森 崇英: "疾病と栄養 14.妊娠と栄養「ベッドサイドの栄養学」糸川嘉則、松倉 茂、清野 裕、編著" 南山堂, 12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Itoh, N.Sagawa, et al.: "Transforming growth factor-beta stimulates, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells" Journal of Clinical Endocrinology and Metabolism. 79(1). 176-182 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Itoh, N.Sagawa, et al.: "Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy" Biochemical and Biophysical Research Communications. 203(1). 602-607 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sagawa, et al.: "Current topic : The role of amniotic endothelin in human pregnancy" Placenta. 15. 565-575 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sagawa, et al.: "Secretion of endothelin by avascular amnion tissue : Possible roles in human pregnancy" Endocrine J.41(Suppl). S117-S125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumiko Inamori, Norimasa Sagawa, et al.: "Activation of phospholipase D in cultured human amnion cells" Reproduction, Fertility and Development. Vol.7. (in press) (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaaki Hasegawa, Norimasa Sagawa, et al.: "Endothelin 1-like immunoreactivity and endothelin receptors in the human placenta from normotensive and hypertensive pregnancies" Journal of Perinatal Medicine. Vol.23. (in press) (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sagawa, et al.: "Biochemical characterristics on phospholipase D in human amniotic membrane" In : Current Progress in Perinatal Medicine, (eds) E.V.Cosmi et al., The Parthenon Publishing Group, New York and London. 401-406 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sagawa, et al.: "Pregnancy and nutrition" In : Diseases and Nutrition, (eds) Y.Itokawa et al.361-372 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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