1996 Fiscal Year Final Research Report Summary
Growth bactor regulation of preimplantation embryo development
| Project/Area Number |
06454477
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
MINAGUTI Hirosi INSTITUTION,DEPARTMENT,TITLE OF POSITION Professor, 医学部, 教授 (20010176)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKAHASI Tuneo Yokohama City Univ. OB / GY,Assist.Prof., 医学部, 講師 (60179497)
TAGA Mitiyosi INSTITUTION,DEPARTMENT,TITLE OF POSITION Associate Prof., 医学部, 助教授 (00107682)
|
|---|
| Project Period (FY) |
1994 – 1996
|
| Keywords | preimplantation / embryo / growth factor / Activin / TGF-beta / RT-PCR |
|---|
| Research Abstract |
Purpose : Our purpose was to clarify the involvement of transforming growth factor-beta (TGF-beta) family in the regulation of preimplantation embryo development.
Methods : The effects of activin-A and TGF-beta on the rates of morula and blastocyst formations as well as on the cleavage velocity of a mouse two-cell embryo in vitro were analyzed. The gene expressions of these two growth factors in various developmental stages were also studied using RT-PCR.
Results : Activin-A at a concentration of 0.2 ng / ml significantly stimulated not only the rate of morula formation but also the velocity of embryo cleavage, whereas no significant effect was found with TGF-beta. RT-PCR revealed that activin-A subunit mRNA,but not TGF-beta mRNA,was detected in preimplantation mouse embryo at any developmental stage.
Conclusions : Activia-A plays an important role in the regulation of preimplantation mouse embryo development in an autocrine fashion.
|
