1995 Fiscal Year Final Research Report Summary
Molecular biological study of inflammatory cytokines in the destruction of periapical lesions.
| Project/Area Number |
06454546
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
TSURUMACHI Tamotsu Nihon University School of Dentistry, Endodontics Assistant Professor, 歯学部, 講師 (60139201)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Periapical lesions / Cytokine / RT-PCR assay / Gene / Molecular biology |
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| Research Abstract |
Candidates stimulators of bone resorption that have been proposed include bacterial components such as lipopolysacharide, prostaglandins, bradykinin, antigen-antibody complexes, and inflammatory cytokines produced by host cells in response to the infection. Inflammatory cytokinesare of particular interest because they are strongly expressed by many host types in response to bacterial infection and they are potent stimulators of bone resorption.
To determine the type of inflammatory cytokines associated with active lesion formation, a molecular biological study was carried out to identify genes. Cytokine gene expression has been analyzed at the level of mRNA.mRNA for inflammatory cytokines was amplified by reverse transcription polymerase chain reaction (RT-PCR).
To identify the bone-destructive mediators, I have analyzed extracts of periapical tissues and periapical exudate cells.
The results were as follows :
1. Immunohistologically, the majority of the T cells infiltrating the periapical lesions was reactive with anti-CD4 antibody.
2. Remarkably high expressions of IL-beta and TNF were observed in all periapical lesions.
3. The expressions of IL-1 alpha, IL-1 beta and TNF were detected in the cells of periapical exudate by RT-PCR assay. The high levels of IL-1 beta correlate with acute apical periodontitis. In addition, when IL-1 alpha and IL-1 beta expressions were compared, IL-1 beta ones were found to be greater than IL-1 alpha ones.
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[Publications] TAKEICHI,O., SAITO,I., TSURUMACHI,T., SAITO,T., MORO,I.: "Human polymorphonuclear leukocytes derived from chronically inflamed tissue express inflammatory cytokines in vivo." Cellular Immunology. Vol.156, No.2. 296-309 (1994)
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