1995 Fiscal Year Final Research Report Summary
Studies of invasive and proliferative activity of oral squamous cell carcinoma
| Project/Area Number |
06454567
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
YAMAMOTO Etsuhide Kanazawa Univ. School of Med. Professor, 医学部, 教授 (00092445)
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| Co-Investigator(Kenkyū-buntansha) |
KUMAGAI Shigehiro Kanazawa Univ. School of Med. Asst. Prof., 医学部・付属病院, 講師 (00215013)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Orthotopic implantaion / Invasion and metastasis / Oral squamous cell carcinoma / Type IV collagen / Desmoglein / Proliferating cell nuclear antigen / Matrix metalloproteinase |
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| Research Abstract |
We devised a new model in order to establish an in vivo model for human oral carcinoma that exhibits significant local invasion and metastasis. In the present study, we examined the invasive sctivity of five kinds of oral squamous carcinoma cells (OSC-19, OSC-20, SS, FA and TT) by this new model. The nude mice had tumor cells from one of five oral squamous cell carcinoma cells implanted into the tongue or the oral floor via an intra-oral route and, as a control, the subcutaneous tissue of the back. The back tumors showed a benign growth pattern, lacking significant invasion of surrounding tissues. In contrast, the tumors implanted into the tongue or the oral floor exhibited invasive growth and stromal reactions, the histological appearance of which was similar to that of the original tumors. Moreover, the regional neck lymph node and pulmonary metastases were observed in this model. Regional neck lymph node metastases were detected in 81.0% of mice implanted with OSC-19 cells. The oral tumor implanted with OSC-19 cells or TT cells showed a cords-like diffuse invasion pattern and was characterized by the abscence of a continuous basement membrane and by a reduction in the intercellular adhesion of carcinoma cells. These results suggest that the invasion of oral squamous cell carcinoma may be influenced by the tumor-stromal and/or intercellular interactions.
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[Publications] Kumagai, S., Imai, K., Nakagawa, K., Yamamoto, E., Nakanishi, I., Okada., Y.: "Immunohistologic distribution of basement membrane in oral squamous cell carcinoma." Head & Neck. 16. 58-63 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Nozaki, S., Endo, Y., Chatani, S., Kumagai, S., Nakagawa, K., Yamamoto, E., Tanaka, M., Sasaki, T.: "Plasminogen activation systemassociated with invasion and metastasis of head and neck tumor." Oral Oncology, Vol.4B : Editor ; Varma, A.K.& Mori, M., Macmillan India Limited. 145-148 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Kawashiri, S., Kumagai, S., Kojima, K., Harada, H., Chatani, S., Yamamoto E.: "Experimental studies on the in vivo model for invasion and metastasis of oral squamous cell carcinoma. Part 1 : Establishment of orthotopic implantation model and investigation of invasive activity." Oral Oncology, Vol.4B : Editor ; Varma, A.K.& Mori, M., Macmillan India Limited. 279-282 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Kojima, K., Kawashiri, S., Kumagai, S., Harada, H., Chatani, S., Yamamoto, E.: "Experimental studies on the in vivo model for invasion and metastasis of oral squamous cell carcinoma. Part 2 : Effect of chemotherapy against invasion and metastasis." Oral Oncology, Vol.4B : Editor ; Varma, A.K.& Mori, M., Macmillan India Limited. 283-286 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Chatani, S., Kawashiri, S., Kumagai, S., Kojima, K., Harada, H., Nozaki, S., Yamamoto E.: "Studies on the invasive activity of DMBA induced tongue carcinoma of the hamsters." Oral Oncology, Vol.4B : Editor ; Varma, A.K.& Mori, M., Macmillan India Limited. 287-290 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Imai, K., Kumagai, S.Nakagawa, K., Yamamoto, E., Nakanishi, I., Okada, Y.: "Immuno-localization of desmoglein and intermediate filaments in human oral squamous cell carcinomas." Head & Neck. 17. 204-212 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Kawashiri, S., kumagai, S., Kojima, K., Harada, H., Yamamoto, E.: "Development of a new invasion and metastasis model of human oral squamous cell c arcinomas." Oral Oncol, Eur J Cancer. 31B. 216-221 (1995)
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「研究成果報告書概要(欧文)」より
