1995 Fiscal Year Final Research Report Summary
Evaluations of apoptosis-relating factors as tumor markers with special reference to diagnosis and treatment in oral cancers
| Project/Area Number |
06454569
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | SAPPORO MEDICAL UNIVERSITY |
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Principal Investigator |
ODAJIMA Tetsuyo Sapporo Medical University Associate Professor, 医学部, 講師 (00177239)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATSUKA Hiroyoshi Sapporo Medical University Associate professor, 医学部, 講師 (50165180)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Oral cancer / Apoptosis / TUNEL / Oncogene protein / Cell proliferation / Clinocopathological factor / Survival / Malignant behavior |
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| Research Abstract |
A total of 93 oral squamous cell carcinomas were analyzed from the viewpoint of apoptotic cell death using in situ nick end-labeling (TUNEL) method and immunohistochemistry for MIB-1 cell proliferation marker and oncoproteins including p53, bcl-2, cyclin D1 and epidermal growth factor (EGFR). The results were correlated with clinicopathological variables and patient out come. The high apoptotic labeling index (AI) was significantly associated with the expressions of MIB-1 and cyclin D1, tumor size, lymph node metastasis, clinical stage, tumor growth pattern and differentiation. Patients with high AI*3.0 and diffuse distribution of apoptotic in cancer cell cell nest showed significantly poorer prognosis than those with low AI<3.0 and localized distribution of them only in the differentiated region. However, Cox hazard multivariate analysis revealed that significant factor influencing malignant potential for oral cancer was only lymph node metastasis, but not apoptosis. These results indicate that apoptosis is followed by cancer cell proliferation or alteration of cell cycle. Analysis of apoptosis in oral cancer is useful in evaluation for biological behavior of oral cancer.
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