Clinical Significance of Platelet Allo-antibodies.

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 06454617
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: University of Tokyo
• Principal Investigator: SHIBATA Yoichi University of Tokyo, Dept.of Transfusion Medicine, Professor, 医学部(病), 教授 (30010474)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Koki Okinaka Memorial Institution Dept.of Medicine, Derector, 主任研究員 (50171484) ; MAEJIMA Masamoto University of Tokyo, Dept.of Transfusion Medicine, Instructor, 医学部(病), 助手 (10251307) ; KUWATA Shoji University of Tokyo, Dept.of Transfusion Medicine, Instructor, 医学部(病), 助手 (00241993)
• Project Period (FY): 1994 – 1995
• Keywords: Platelet Allo-antigen / Platelet Allo-antibody / Platelet Transfusion / Neonatal Alliommune Thrombocytopenia

Research Abstract

1) We were unable to detect Mo^a, Sr^a or Va^a antigens among Japanese using our platelet-panel. In contrast, about 5% (15/314) were found to be Tu^a positive.
2) We have experienced 29 cases of NAIT (Neonatal Alloimmune Thrombocytopenia) : 20 cases due to anti-HPA-4b (YuK^a) antibodies, 3 cases due to anti-HPA-4a (Yuk^b), 5 cases due to anti-HPA-3a (Bak^a) and 1 case due to anti-HPA-5b (Br^a). Among them 3 cases were complicated with intracranial hemorrhage.
3) By screening a large number of samples, we found that anti-HPA-2b (Sib^a) playd an important role in the case of platelet transfusion refractoriness.
4) The MPHA (mixed passive hemagglutination) method was developed about 15 years ago as a simple and sensitive test for the detection of anti-platelet antibodies. It has been refined now. Using 5% sucrose, the platelet-fixed plate can be kept frozen (below-40 C) without loss of reactivities for more than a year. Thus, MPHA should be employed as the routine test in any lab in the hospital for the detection of platelet allo-antibodies.

Research Products

• [Publications] Tanaka S, et al: "Genotype frequencies of the human platelet antigen, Ca/Tu, in Japanese,determined by a PcR-RFLP method" Vox Sang. 70. 40-44 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsui K et al: "Preinatal Intracranial Hemorrhage due to Severe Neonatal Alloimmune Thtombocytopenic Purpura(NAITP)Associated with Anti-Yuk^b(HPA-4a)Antibodies." Brain and Development. 17. 352-355 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Araki N et al: "Anti-HLA Antibody Screeing with Platelet Soluble HLA Antigens by MPHA Method." VOX Sang. 69. 222-230 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fujiwara K et al: "DNA-Based Typing of Human Platelet Antigen Systems by PCR-SSCP(Songle-Strand-Conformation Polymorphism)Method." VOX Sang. 69. 347-351 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Lin M et al: "Neonatal Alloimmune Thrombocytopenia in Taiwan Due to an Antibody against a Labile Component of HPA-3a(Bak^a)" VOX Sanginis. 69. 336-340 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsui K., Ohsaki E., Goto A., Koresawa M., Kigasawa Y., Shibata Y.: "Perinatal Intracranial Hemorrhage due to Severe Neonatal Alloimmune Thrombocytopenic Purpura (NAITP) Associated with Anti-Yuk^b (HPA-4a) Antibodies." Brain and Development. 17. 352-355 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Araki N., Shibata Y., Inada H., Nose Y., Sakata N., Ito K.: "Anti-HLA Antibody Screening with Platelet Soluble HLA Antigens by MPHA Method." Vox Sang. 69. 222-230 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fujiwara K., Tokunaga K., Isa K., Miyamoto M., Wang L., Akaza T., Tadokoro K., Shibata Y., Juji T.: "DNA-Based Typing of Human Platelet Antigen Systems by PCR-SSCP (Single-Strand Conformation Polymorphism) Method." Vox Sang. 69. 347-351 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Lin M., Shieh S., Liang D., Yang T., Shibata Y.: "Neonatal Alloimmune Thrombocytopenia in Taiwan Due to an Antibody against a Labile Component of HPA-3a (Bak^a)." Vox Sanginis. 69. 336-340 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tanaka S., Taniue A., Nagao N., Ohnoki S., Tomita T., Shibata H., Okubo Y., Yamaguchi H., Shibata Y.: "Genotype frequencies of the human platelet antigen, Ca/Tu, in Japanese, determined by a PCR-RFLP method" Vox Sang. 70. 40-44 (1996)
  • Description: 「研究成果報告書概要(欧文)」より