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1995 Fiscal Year Final Research Report Summary

Analgsis of Bioligical Functions of Ganglisides

Research Project

Project/Area Number 06454657
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionFACULTY OF AGRICULTURE

Principal Investigator

ITO Makoto  INSTTIUTION KYUSHU UNIVERSITY DEPARTMENT AGRICULTURE TTILE OF POSTTION ASSOCIATEPROFESSOR, 農学部, 助教授 (40253512)

Co-Investigator(Kenkyū-buntansha) HIGASHI Hideyoshi  INSTTIUTION MITSUBISHIKASEI OF LIFESCIENCES TTILE OF POSTTION SENIOR RESEARCHER, 主任研究員
Project Period (FY) 1994 – 1995
KeywordsGanglioside / Endogly coceramidase / Glycolipid / Ceramide / Neuron / Cam kII / Calmodulin / GMI
Research Abstract

This report describes the homeostasis of glycosphingolipid (GSL) on the cell surface as revealed for the first time by an application of endoglycoceramidase (EGCase) capable of hydrolyzing the linkage between the oligosaccharide and the ceramide of various GSLs. When cell-surface GSLs of B16 melanoma cells were hydrolyzed by the action of EGCase, the synthesis of GSLs was found to increase transiently, possibly due to the activation of UDP-glucose : ceramide glucosyltransferase. As a result, the cell-surface GSL content was restored quickly to exactly the same level found without the EGCase treatment, if EGCase was removed from the cell culture. Treatment of erythrocytes with EGCase was found to increase the ceramide content of the plasma membrane. Surprisingly, however, in B16 cells the increase of membrane ceramide by EGCase caused the suppression of de novo ceramide production, resulting in maintenance of the ceramide content of B16 cells at the same level even after EGCase treatment. The signal for homeostatic regulation could be the ceramide released by the action of EGCase, since C_2-ceramide was found to mimic in part the action of EGCase ; it suppressed de novo production of ceramide and was directly converted to C_2-ceramide GM3. Our finding demonstrates a novel form of homeostatic regulation coupled to the GSL-synthesizing system in mammalian cells for maintaining the contents of both cell-surface GSLs and free ceramide. Since many opportunistic pathogens were found to produce EGCase extracellularly, this restoration mechaniam could also be present as a defense mechanism against microbial EGCase.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] MAKOTO ITO: "A novel form of homeostatic regulation in B16 melanoma for maintaing the confents of both cell-surface GSLs and ceramids." J. Biol. Chem.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MAKOTO ITO: "A novel enzyme that cleaves the N-acyl linkage of ceramides in various GSLs as well as sphimgomyelin to produce their lgso forms" J. Biol. Chem.270. 24370-24374 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIRONOBU KOMORI: "COnversion of chort-chain ceramides to short-chain ceramide GM3 in B16 melanoma cells" FEBS Leters. 374. 299-302 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] LI JI: "The hydrolysis of cell-surface GM3 by EGCase reduces EGFR phosphorylation in A431 cells" Glycobiology. 5. 343-350 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KAZUYO MURAMOTO: "Endoglycocetamidase treatment inhibits synchronous oacillations of intracellular Ca^<2+> in cultured cortical neurons." Biochem. Biophy. Res. Commun.202. 398-402 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MICHIO SHIMAMURA: "Repulsive contribution of surface sialic acid residues to cell adhesion to substratum" Biochem. Molecular Biol. Inter.33. 871-878 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊東 信: "ガングリオシド研究法 II" 鈴木康夫,安藤進編著 学会出版センター, 273 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimamura, M., Shibuya, N.Ito, M.and Yamagata, T.: "Repulsive contribution of surface sialic acid residues to cell adhesion to substratum." Biochem. Molecular Biol.Inter. 33. 871-878 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muramoto, K., Kawahara, M., Kobayashi, K., Ito, M., Yamagata, T.and Kuroda, Y.: "Endoglycoceramidase tratment inhibits synchronous oscillations of intracellular Ca^<2+> in cultrued cortical neurins." Biochem.Biophy.Res.Commun.202. 398-402 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ji, L., Ito, M., Zhang, G.and Yamagata, T.: "The hydrolysis of cell surface glycosphingolipids by endoglycoceramidase reduces epidermal growth factor receptor phophorylation in A431 cells." Glycobiology. 5. 343-350 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, M., Kurita, T.and Kita, K.: "A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as spingomyelin to produce their lyso forms." J.Biol.Chem.270. 24370-24374 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komori, H.and Ito, M.: "Conversion of short-chain ceramides to short-chain ceramide GM3 in B16 melanoma cells." FEBS Lett. 374. 299-302 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagatuka, Y., Watarai, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y.: "Production of human monoclonal antibodies to iblood group by EBV-induced transformation : Possible presence of a new glycolipid in cord red cell membranes and human hematopoietic cell lines." Immunology Lett.46. 93-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, M.and Komori, H.: "A novel form of homeostatic regulation in B16 melanoma cells for maintaining the contents of both cell-surface glycosphingolipids and ceramide." J.Biol.Chem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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