1995 Fiscal Year Final Research Report Summary
Molecular Mechanism of Signal Transduction in Prokaryotes
| Project/Area Number |
06454670
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
MIZUNO Takeshi Nagoya University, School of Agricalture, Professor, 農学部, 教授 (10174038)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Escherichia coli / Signal transduction / Protein phosphorylation / ArcB sensor / Signaling network |
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| Research Abstract |
Recently we demonstrated the occurrence of a novel device of signal transducers in Escherichia coli. Among such E.coli signal transducers, the arcB is of particular interest, because these proteins are unique in the sense that they contain both an autophosphorylated histidine site (or transmitter module) and a phospho-accepting aspartate site (or receiver module) in their primary amino acid sequences. ArcB was originally characterized as the signal transducer involved in anaerobic repression of regulons of the aerobic function. We demonstrated that ArcB possesses in the C-terminal region another phosphorylated histidine site, in addition to the previously indentified authentic one. It was demonstrated for ArcB that the C-terminal domain containing the histidine (His-717) can function as an alternative phosphodonor (a truncated form of ArcB containing this C-terminal region was previously designated as ArcB^C). This novel type of sensory kinases was revealed to contain two phosphorylated histidine sites together with a phospho-accepting asparatate site. It is therefore most likely that ArcB can make a complex phosphotransfer circuitry. Furthermore, our resuls supported the view that ArcB can serve as a powerful device for not only propagating multi-signals but also making up signaling networks through more sophisticated manners than thought previously.
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[Publications] Kondo, H., Miyaji, T., Suzuki, M., Tate, S., Mizuno, T., Nishimura, Y., Tanaka, I: "Crystallization and X-ray studies of DNA-binding domain of OmpR protein ; a positive regulator involved in activation of osmoregulatory genes in Escherichia coli." Mol.Biol.235. 780-782 (1994)
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[Publications] Yamada, H., Mori, H., Momoi, H., Nakagawa, Y., Ueguchi, C., Mizuno, T: "A fission yeast gene encoding a protein that preferentially binds to curved DNA" Yeast. 10. 883-894 (1994)
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[Publications] Shindo, H., Iwaki, T., Ieda, R., Kurumizaka, H., Ueguchi, C., Mizuno, T.Morikawa, S., Nakamura, H., Kubpniwa, H: "Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS,from Escherichia coli." FEBS Lett. 360. 125-131 (1995)
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[Publications] Ohmiya, R., Yamada, H., Nakashima, K., Aiba, H., Mizuno, T: "Osmoregulation of fission yeast : cloning of two distinct genes encoding glycerol-3-phosphate dehydrogenase, one of which is responsible for osmotolerance for growth." Mol.Microbiol.18. 963-973 (1995)
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