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1996 Fiscal Year Final Research Report Summary

Development of an Analyzing System of the Cellular Responses to Multiple Stresses

Research Project

Project/Area Number 06555245
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 生物・生体工学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUOKA Hideaki  Tokyo University of Agriculture and Technology, Faculty of Technology Professor, 工学部, 教授 (10143653)

Co-Investigator(Kenkyū-buntansha) KAWANA Akio  NTT Basic Research Laboratories, Material Science Research Laboratory, Kawana Re, 物質科学研究部, 川名研究グループリー
HOMMA Tomoo  Tokyo University of Agriculture and Technology Faculty of Technology, Research A, 工学部, 助手 (80242246)
NEMOTO Yasuyuki  Tokyo University of Agriculture and Technology, Faculty of Technology, Lecturer, 工学部, 講師 (70202249)
Project Period (FY) 1994 – 1996
Keywordsmulti-channnel sub-micron capillary / stress signal / cellular response to stress / chemical stress signal / electrical stress signal
Research Abstract

Microbioassay for stress signal to living cells has recently been recognized important from the viewpoint new drug development and clinical analysis. This study aimed to develop an analyzing system of cellular responses to multiple stress signals. Multi-channnel sub-micron capillary (MMC) was used to apply a chemical or electrical stress signal to a particular plant cell and, at the same time, to detect the response signal of the cell or another cell. The response signals were chemicel, electrical, or electrochemical.
Anionic fluorescence dye, lucifer yellow (LY), was introduced into a cell of cultured tobacco (BY-2, derived from Nicotiana tabacum L.cv.Bright Yellow-2) or a leaf cell of Tradescantia virginiana L.as an example of a chemical signal. The diffusion pattern of LY was found a useful indicator to confirm the tip position of the capillary. In the same way, H^+, K^+, Cl were injected in a cell and its injection was ascertained with respective ion-selective microelectrodes which were devised with MMC.The reaction of enzymes such as luciferase and urease which were injected in a cell was also measured in vivo.
In order to estimate an exact voltage acting between the intercellular membrane, a high performence impedance analyzing system was developed using a patch-clamp amplifier and a lock-in amplifier. The membrane impedance of a intercellular membrane of BY-2 was, for example, determined as a parallel circuit of 2-7G OMEGA and 500pF.
The present system has enabled the application of multiple stress signals separately or simultaneously, or repeatedly to the same single cell. This is a marked advantageous point in studying complicated relationships between stress signal intensity and cellilar response intensity.

  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] Tomoo Homma: "Analysis of Electric and Electrochemical Connection Between Leaf Cells of Tradescantia virginiana L. That Responds to CO_2 Stress by Changing Intracellular Potential" Bioelectrochem.Bioenerg.33. 67-70 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Initiating Activity" J.Intell.Matl.Systs.& Struct.5. 117-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to Several Antifungal Agents Treated as Short-Term Stress" Microbiology. 140. 2409-2414 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "植物の環境認識機能" システム/制御/情報. 38. 485-491 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "植物センサーの働き" ESTaP. 27. 8-11 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ki-Bong Oh: "Transmission of the Effect of an Antifungal Agent within a Single Hypha." Appl.Microbiol.Biotechnol.44. 473-478 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Matsuoka: "Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol.36. 19-24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by an Ionic Intensified Fluorescein Diacetate Method." Current Microbiol.30. 173-176 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hyeong-Cheol Yang: "Application of Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity." J.Antibacterial Antifungal Agent. 23. 3-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hyphal Growth." Appl.Microbiol.Biotechnol.43. 102-108 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ki-Bong Oh: "Combined Effect of Amphotericin B and Flucytosine on Hyphal Growth of Candida albicans Estimated at a Single Hypha Level." J.Med.Vet.Mycol.33. 191-195 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "農業環境における植物の細胞センシング" 農業機械学会誌. 58. 120-125 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jong-Chul Park: "Influence of Antifungal Pre-treatment in Preparing Test Mycelium on MIC Values of Several Antifungal Agents against Aspergillus niger" Mycoscience. 37. 117-120 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 呉 基鳳: "バイオセンシング技術の展望" 防菌防黴. 23. 487-494 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 斉藤美佳子: "微小電極を用いた細胞電位の測定" DENKI KAGAKU. 64. 107-111 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Matsuoka: "Sensitive Detection of Antifungal Activity of Volatile Components Emitted from Plant Leaf Samples by Single Hypha Assay Method" Biocontrol Science. 1. 65-67 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshioka: "Intracellular Fate of 2-NBDG,A Fluorescent Probe for Glucose Uptake Activity,in Escherichia Coli Cells" Biosci.Biotech.Biochem. 60. 1899-1901 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshioka: "Evaluation of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose,A New Fluorescent Derivative of Glucose,for Viavility Assessment of Yeast Candida albicans" Appl.Microbiol.Biotechnol.46. 400-404 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikako Saito: "Application of Multifunctional Microelectrode to the In Vivo Analysis of the K^+ Response to Co_2 Stress" Bioelectrochem.Bioenerg.39. 115-118 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ki-Bong Oh: "Morphological Recognition of Fungal Spore Germination by a Computer-aided Image Analysis and Its Application to Antifungal Activity Evaluation" J.Biotechnology. 45. 71-79 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikako Saito: "Microinjection of Fluorescence Dye in a Plant Cell and Its Intercellular Translocation with a Multi-channel Microelectrode System" Biochim.Biophys.Acta. 1289. 1-4 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshioka: "A Novel Fluorescent Derivative of Glucose Applicable to the Assessment of Glucose Uptake Activity of Escherichia coli" Biochim.Biophys.Acta. 1289. 5-9 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ki-Bong Oh: "Rapid and Sensitive Screening of Antifungal Activity in Medicinal Plants by Single Cell Biosensing System" Biosci.Biotech.Biochem.60. 911-913 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to acidic conditions and Its Relation to Salt Stress and Miconazole" Mycological Research. 100. 869-874 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "植物バイオセルの計測 -植物におけるシステミック応答機構の解明をめざして-" 蛋白質 核酸 酵素. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomoo Homma: "Analysis of Electric and Electrochemical Connection Between Leaf Cells of Tradescantia virginiana L.That Responds to CO_2 Stress by Changing Intracellular Potential" Bioelectrochem.Bioenerg.33. 67-70 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Initiating Activity" J.Intell.Matl.Systs.& Struct.5. 117-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to Several Antifungal Agents Treated as Short-Term Stress" Microbiology. 140. 2409-2414 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ki-Bong Oh: "Transmission of the Effect of an Antifungal Agent within a Single Hypha." Appl.Microbiol.Biotechnol.44. 473-478 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ki-Bong Oh: "Combined Effect of Amphotericin B and Flucytosine on Hyphal Growth of Candida albicans Estimated as a Single Hypha Level." J.Med.Vet.Mycol.33. 191-195 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hyphal Growth." Appl.Microbiol.Biotechnol.43. 102-108 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by an Ionic Intensified Fluorescein Diacetate Method." Current Microbiol.30. 173-176 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki matsuoka: "Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol.36. 19-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hyeong-Cheol Yang: "Application of Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity" J.Antibacterial Antifungal Agent. 23. 3-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka: "Sensitive Detection of Antifungal Activity of Volatile Components Emitted from Plant Leaf Samples by Single Hypha Assay Method" Biocontrol Science. 1. 65-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshioka: "Intracellular Fate of 2-NBDG,A Fluorescent Probe for Glucose Uptake Activity, in Escherichia coli Cells" Biosci.Biotech.Biochem.60. 1899-1901 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshioka: "Evaluation of 2- [N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino] -2-deoxy-D-glucose, A New Fluorescent Derivative of Glucose, for Viability Assessment of Yeast Candida albicans" Appl.Microbiol.Biotechnol.46. 400-404 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito: "Application of Multifunctional Microelectrode to the In Vivo Analysis of the K^+ Response to CO_2Stress" Bioelectrochem.Bioenerg.39. 115-118 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ki-Bong Oh: "Morphological Recognition of Fungal Spore Germination by a Cmputer-aided Image Analysis and Its Application to Antifungal Activity Evaluation" J.Biotechnology. 45. 71-79 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito: "Microinjection of Fluorescence Dye in a Plant Cell and Its Intercellular Translocation with a Multi-channel Microelectrode System" Biochim.Biophys.Acta. 1289. 1-4 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshioka: "A Novel Fluorescent Derivative of Glucose Applicable to the Assessment of Glucose Uptake Activity of Escherichia coli" Biochim.Biophys.Acta. 1289. 5-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ki-Bong Oh: "Rapid and Sensitive Screening of Antifungal Activity in Medicinal Plants by Single Cell Biosensing System" Biosci.Biotech.Biochem.60. 911-913 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to acidic conditions and Its Relation to Salt stress and Miconazole"

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mycological Research. 100. 869-874 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jong-Chul Park: "Influence of Antifungal Pre-treatment in Preparing Test Mycelium on MIC Values of Several Antifungal Agents against Aspergillus niger" Mycoscience. 37. 117-120 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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