1996 Fiscal Year Final Research Report Summary
Breeding of the Soybean Lines with High Quality Edible Oil Utilizing a Lipoxigenase-Lacking Trait in Seed
| Project/Area Number |
06556001
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 試験 |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | Iwate University |
|---|
Principal Investigator |
KAIZUMA Norihiko Iwate University, President, 教授 (00003773)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Keisuke Agric.Res.Center.Min.Agric.Forest.& Fish., Lavoratory Chief, 農業研究センター, 室長
|
|---|
| Project Period (FY) |
1994 – 1996
|
| Keywords | Artificial Mutation Induction / Soybean Seed Proteins / Lipoxigenase / Edible Oil |
|---|
| Research Abstract |
The ultimate objective of this study is to develope completely lipoxigenase-lacking soybean lines in seeds which are characterized with oil deterioration-resistance by effect of no or less oxidation of non-suturated fatty acids.
Some promising soybean F3 lines were selected from the hybridization between Kyushu 11 (lypoxigenase-lacking) and high oil Jilin 11/Williams in the Agricutural Research Center. However, oil analyzes and oil-deterioration tests are now in progress.
On the other hand, the soybean lines accumulating some seed protein-subunit lacking variant genes (7S and 11S globulin-subunits) through hybridizations were developed in Iwate University for future hybridization programs with lipoxigenade-lacking lines. SDS-PAGE analyzes using about 3,500 F2 seeds were carried out for genetic analyzes of the protein-subunit lacking traits. A part of them were used for oil and protein contents analyzes and very interesting results were obtained.
Electron microscopic observations of the protein bodies and spherosomes in the 7S and/or 11S globulin subunit-lacking variants brought some interesting results.
|
