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1996 Fiscal Year Final Research Report Summary

Measurement of intracellular signaling events induced by cell-cell interactions in a live organ.

Research Project

Project/Area Number 06557024
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Immunology
Research InstitutionScience University of Tokyo

Principal Investigator

NAKAYAMA Toshinori  Science University of Tokyo, Research Institute for Biological Sciences, Associate Professor, 生命科学研究所, 助教授 (50237468)

Co-Investigator(Kenkyū-buntansha) SUZUKI Kazuo  National institute of Health Japan, Researcher, 研究員 (20192130)
Project Period (FY) 1994 – 1996
Keywordscalcium / oscillation / コンピューター画像解析
Research Abstract

We have tried to established a system, in which intracellular signaling event are quantitatively detected and visualized on the computer displays. The materials used are lymphocytes, thymocytes, and thymic organs. Those cells were atimulated with anti-T cell antigen receptor antibodies or PMA & ionomycin. The intra-cellular free calcium ion concentration ([Ca2+]i) was measured in the stimulated cells for 15 min.to 1 hour. We could detect a rapid increase and long-lasting oscillations of [Ca2+]i quantitatively. These data obtained by using this grant are published in several journals. The measurement was also succeeded in thymocytes presented in the organ cultured thymus. This assasy system is one of the novel experimental detection system, and provide a possibility for analyzing intra-cellular signaling events in live organs.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Benveniste,P.: "Engagement of the External Domains of CD45 Tyrosine Phosphatase can Regulate the Differentition of Immature CD4^+CD8^+ Thymocytes into Mature T-Cells" Proc.Natl.Acad.Soi.USA. 91. 6933-6937 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kearse.K.P.: "Developmental Regulation of α-β-T-cell Antigen Receptor Expression Results from Differential Stability of Nescent TCR-α Proteins within the Endoplasmic-Reticulum of Immature and Mature T-Cells" EMBO Journal. 13. 4504-4514 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wange,C.R.: "T-Cell Receptor-Mediated Signaling Events in CD4^+CD8^+ Thymocytes Undergoing Thymic Selection - Requirement of Calcineurin Activation for Thymic Positive Selection but not Negative Selection" J.Exp.Med.181. 927-941 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kishimoto,H.: "Physical Dissociation of the TCR-KD3 Complex Accompanies Receptor LIgation" J.Exp.Med.182. 1997-2006 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto,K.: "Requirement for P56(LCK) Tyrosine kinase Activation in T-Cell Receptor-Mediated Thymic Selection" J.Esp.Med. 184. 931-943 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 橋本香保子: "免疫学への招待" 南江堂, 163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,T.: "Handback of Fiperiental" Blackwell Scientific Publications(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Benveniste, P., Takahama, Y., Wiest, D.L., Nakayama, T., Sharrow, S.O.and Singer A.: "Engagement of the external domains of CD45 tyrosine phosphatase can regulate the differentiation of immature CD4^+CD8^+ thymocytes into mature T cells." Proc.Natl.Acad.Sci.USA. 91. 6933-6937 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kearse, K.P., Roberts, J.L., Munitz, T.I., Wiest, D.L., Nakayama, T., and Singer A.: "Developmental regulation of alphabeta T cell antigen receptor expression results from differential stability of nascent TCRalpha proteins within the endoplasmic reticulum of immature and mature T cells." EMBO Journal. 13. 4504-4514 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wang, C.-R., Hashimoto, K., Kubo, S., Yokochi, T., Kubo M., Suzuki M., Suzuki K., Tada T., and Nakayama, T.: "TCR-mediated signaling events in CD4^+CD8^+ thymocytes undergoing thymic selection : Requirement of calcineurin activation for thymic positive selection but not negative selection." J.Exp.Med.181. 927-941 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kishimoto H., Kubo R.T., Yorifuji, H., Nakayama, T.Asano, Y., and Tada, T.: "Physical dissociation of the TCR/CD3 complex accompanies receptor ligation" J.Exp.Med.182. 1997-2006 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, T.: "T cell development in the thymus. Handbook of Experimental Immunology. (5th ed.) Edited by V.Reeders." Blackwell Scientific Publications. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, K., Sohn, S.J., Levin, S.D., Tada, T., Perlmutter, R.M., and Nakayama, T.: "Requirement for p56^<lck> tyrosine kinase activation in TCR-mediated thymic selection." J.Exp.Med.184. 931-943 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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