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1996 Fiscal Year Final Research Report Summary

Genes involved in insulin secretion and improvement of detection method for gene mutations in diabetic patients

Research Project

Project/Area Number 06557056
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 内分泌・代謝学
Research InstitutionYamaguchi University School of Medicine

Principal Investigator

OKA Yoshitomo  Yamaguchi University School of Medicine Professor, 医学部, 教授 (70175256)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Yasuo  Yamaguchi University Hospital Resident, 医学部・附属病院, 医員
TAKEUCHI Hideo  Yamaguchi University Hospital Resident, 医学部・附属病院, 医員
UEDA Kouhei  Yamaguchi University Hospital Resident, 医学部・附属病院, 医員
Project Period (FY) 1994 – 1996
Keywordsdiabetes mellitus / insulin secretion / mitochondrial gene / gene mutation / dot blot hybridization
Research Abstract

To investigate the prevalence and clinical characteristics of diabetes mellitus caused by mitochondrial gene mutations in the tRNALeu (UUR), we screened 440 diabetic patients with diabetic mothers for mitochondrial gene mutations at nucleotide pair (np) 3250,3256,3260,3271 and 3291 in addition to an A to G transition at up 3243. The dot-blot hybridization method using 32P-labeled sequence-specific oligonucleotides (SSO) as probes was employed in this study. While no diabetic patients having a point-mutation at np 3250,3256,3260 or 3291 were found, one carrying a T to C transition at np 3271 and seven carrying an A to G transition at 3243 were identified. The patient with the 3271 mutation, a 39-year-old male, had excellent glycemic control with diet therapy alone and had neither hearing impairment nor symptoms suggesting mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS). In summary, among diabetic patients, the prevalence of the 3271 mutation was about 14% that of the 3243 mutation, while other mutations in the tRNALeu (UUR) are even more rare in maternally transmitted diabetes.
We have also cloned the gene involved in glucose-induced insulin secretion from pancreatic beta cells, hGIRK2 and mGPDH,and analyzed the mutations of these genes in Japanese diabetic patients.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Katagiri H,et al.: "Mitochondrial diabetes mellitus : prevalence and clinical characterization of diabetes due to mitochondrial tRNA^<Leu (UUR) > gene mutation in Japanese." Diabetologia. 37. 504-510 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara H,et al.: "Human GLUT-2 overexpression does not affect glucose-stim ulated insulin secretion in MIN6 cells." Am.J.Physiol.269. 897-902 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanizawa Y,et al.: "A human pancreatic lslet inwardly rectifying potassium channel : cDNA cloning,determination of the genomic structure and genetic variations in Japanese NIDDM patients." Diabetologia. 39. 447-452 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara H,et al: "Effect of mitochondrial and/or cytosolic glycerol 3-phosphate dehydrogenase overexpression on glucose-stimulated insulin secretion from MIN6 and HIT cells." Diabetes. 45. 1238-1244 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oka Y,et al.: "β-cell loss and glucose induced signalling defects in diabetes mellitus caused by mitochondrial tRNA^<Leu (UUR) >gene mutation." Diabetic Medicine. 13. 98-102 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi Y,et al: "Detection of variants in the mitochondrial glycerophosphate dehydrogenase gene in Japanese NIDDM patients." Diabetologia. (in Press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katagiri H,Asano T,Ishihara H,Inukai K,anai M,Yamanouchi T,Tsukuda K,Kikuchi M,Kitaoka H,Ohsawa N,Yazaki Y,and Oka Y.: "Mitochondrial diabetes mellitus : prevalence and clinical characterization of diabetes due to mithochondrial tRNALeu (UUR) gene mutation in Japanese." Diabetologia. 37. 504-510 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara H,Asano T,Tsukuda K,Katagiri H,Inukai K,Anai M,Yazaki Y,Miyazaki J-I,Kikuchi M and Oka Y.: "Human GLUT-2 overexpression does not affect glucose-stimulated insulin secretion in MIN6 cells." Am.J.Physiol.269. 897-902 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanizawa Y,Matsubara A,Ueda K,Katagiri H,Kuwano A,Ferrer J,Permutt MA and Oka Y.: "A human pancreatic lslet in wardly rectifying potassium channel : cDNA cloning, determination of the genomic structure and genetic variations in Japanese NIDDM patients." Diabetologia. 39. 447-452 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi T,Oka Y,Katagiri H,Falorni A,Kasuga A,Takei I,Nakanishi K,Murase T,Kosaka K and Lernmark A.: "Association between HLA and islet cell antibodies in diabetic patients with a mitochondrial DNA mutation at base pair 3243." Diabetologia. 39. 1196-1200 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara H,Nakazaki M,Kanegae Y,Inukai K,Asano T,Katagiri H,Yazaki Y,Kikuchi M,Miyazaki J,Saito I and Oka Y.: "Effect of mitochondrial and/or cytosolic glycerol 3-phosphate dehydrogenase overexpression on glucose-stimulated insulin secretion from MIN6 and HIT cells." Diabetes. 45. 1238-1244 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara H,Shibasaki Y,Kizuki N,Katagiri H,Yazaki Y,Asano T and Oka Y.: "Cloning of cDNAs encoding two isoforms of 68-kDa type I phosphatidylinositol 4-phosphate 5-kinase from insulin secreting cells." J Biol Chem. 271. 23611-23614 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oka Y,Katagiri H,Ishihara H,Asano T,Kobayashi T and Kikuchi M.: "beta-cell loss and glucose induced signalling defects in diabetes mellitus caused by mitochondrial tRANLeu (UUR) gene mutation." Diabetic Medicine. 13. 98-102 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi Y,Matsutani A and Oka Y.: "Detection of variants in the mitochondrial glycerophosphate dehydrogenase gene in Japanese NIDDM patients." Diabetologia. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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