• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Factors affecting the efficiency of protein secretion in E.coli.

Research Project

Project/Area Number 06558096
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionInstitute of Molecular and Cellular Biosciences, University of Tokyo

Principal Investigator

TOKUDA Hajime  University of Tokyo, Institute of Molecular and Cellular Biosciences, Professor, 分子細胞生物学研究所, 教授 (40125943)

Co-Investigator(Kenkyū-buntansha) MATSUYAMA Shin-ichi  University of Tokyo, Institute of Molecular and Cellular Biosciences, Associate, 分子細胞生物学研究所, 助教授 (50183108)
Project Period (FY) 1994 – 1995
KeywordsOverproduction of Sec factors / Protein secretion / E.coli / Chaperone / Precursor protein / SecG / Everted membrane vesicles
Research Abstract

Preprotein translocase of E.coli comprises a peripheral component, SecA,and integral membrane components, SecY,SecE and SecG.SecA is thought to deliver the preprotein to the putative protein-conducting channel formed by SecY and SecE by undergoing ATP-driven cycles of membrane insertion and deinsertion. SecG renders the translocase highly efficient by unknown mechanism. Preprotein translocation into everted membrane vesicles is inhibited by an externally added antibody raised against the C-terminal region of SecG.However, we found that this region is exposed to the inside (periplasmic side) of membrane vesicles in the absence of preprotein translocation, thereby being protected from external proteinase K.Surprisingly, when preprotein translocation was started with ATP hydrolysis, the C-terminal region was exposed to the outside (cytoplasmic side) of membrane vesicles and thus digested by proteinase K.Another region of SecG showed a change in membrane sidedness, from the cytoplasmic to the periplasmic side, upon preprotein translocation, indicating that SecG undergoes topology inversion. This topology inversion was tightly coupled to the SecG function, and linked with the insertion-deinsertion cycle of SecA.We propose here that the inversion cycle of the SecG topology facilitates the insertion-deinsertion cycle of SecA,thereby causing efficient preprotein translocation.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Tokuda, H.: "Biochemical characterization of the presecretory protein translocation machinery of Escherichia coli." FEBS Lett.(Minireview). 346. 65-68 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishiyama, K.: "Disruption of the gene encoding p12(SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature." EMBOJ.13. 3272-3277 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Douville, K.: "Band 1 subunit of Escherichia coli preprotein translocase and integral membrane export factor p12 are the same protein.(Communication)" J. Biol. Chem.269. 18705-18707 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanada, M.: "Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG(p12)." J. Biol. Chem.269. 23625-23631 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama, S.: "A novel periplasmic carrier protein involved in the sorting and transport of E. coli lipoproteins destined for the outer membrane." EMBOJ.14. 3365-3372 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishiyama, K.: "Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation." Cell. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kontinen, V. P.: "Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in a secG deletion mutant of Escherichia coli at low temperature." FEBS Lett.364. 157-160 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishiyama, K.: "Preferential interaction of SecG with SecE stabilizes an unstable SecE derivative in the Escherichia coli cytoplasmic membrane." Biochem. Biophys. Res. Commun.217. 217-223 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanada, M.: "SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low temperature." FEBS Lett.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokuda, H: "Biochemical characterization of the presecretory protein translocation machinery of Escherichia coli." FEBS Lett.(Minireview). 346. 65-68 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishiyama, K: "Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature." EMBO J. 13. 3272-3277 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Douville, K: "Band 1 subunit of Escherichia coli preprotein translocase and integral membrane export factor p12 are the same protein. (Communication)" J.Biol.Chem. 269. 18705-18707 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanada, M: "Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY,SecE and SecG (p12)." J.Biol.Chem. 269. 32625-23631 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kontinen, V.P: "Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in a secG deletion mutant of Escherichia coli at low temperature." FEBS Lett. 364. 157-160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, S.: "A novel periplasmic carrier protein involved in the sorting and transport of E.coli lipoproteins destined for the outer membrane." EMBO J.14. 3365-3372 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishiyama, K: "Preferential interaction of SecG with SecE stabilizes an unstable SecE derivative in the Escherichia coli cytoplasmic membrane." Biochem.Biophys.Res.Commun. 217. 217-223 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishiyama, K: "Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation." Cell. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanada, M: "SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low low temperature." FEBS Lett. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi