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1996 Fiscal Year Final Research Report Summary

Manufacture of Scanning Force Microscope for Biological Research

Research Project

Project/Area Number 06558099
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Biophysics
Research InstitutionKanazawa University

Principal Investigator

ANDO Toshio  Kanazawa University, Science, Professor, 理学部, 教授 (50184320)

Co-Investigator(Kenkyū-buntansha) TAKENOBU Takayoshi  Olympus, 2nd R/D,Researcher, 第2開発部, 研究員
HAYASHI Yoshiaki  Olympus, 2nd R/D,Chief, 第2開発部, 係長
Project Period (FY) 1994 – 1996
KeywordsAtomic Force Microscope / AFM / High-speed Scanner / High-speed Scanning / Protein / Bi-molecular Interaction / Myosin Heads
Research Abstract

(a) We manufactured an atomic force microscope that was able to be scanned faster and less susceptible to drift than ordinary AFM apparatuses. This AFM was attached to an epiluminescence fluorescence microscope. To show the high performance of our AFM apparatus we tried to obtain high resolution images of myosin heads (subfragment-1) in aqueous solution. The images revealed fine structures that was comparable to those in an atomic model of S1 derived from x-ray crystallography of S1. In the AFM images we were able to see the ATP binding site as well as the large cleft at the tip of S1. These results proved that our AFM apparatus was on the level of the greatest in the world.
(b) We performed a study preparatory to the development of a high-speed AFM in which one image would be acquired within 30msec. We designed and manufactured a high-speed scanner and attained high-speed imaging where one image was obtained within 150msec. By way of experiment we produced an AFM that employed a displacemant detection system suitable for high-speed imaging. We also examinds methods for preparing cantilevers that were suitable for a high-speed AFM.From these preparative studies we were able to secure a scaffolding for the development of the video-rate AFM.
(c) To measure bimolecular interaction force at a truly single molecular level we developed a method to capture a single molecule of protein at the apex of a cantilever tip. Using this method we succeeded in quantifying the force field exerted between a single molecule of heavy meromyosin (HMM) and actin. Using this new technology we also discovered that ATP binding to HMM heads induced vibratile structural changes in HMM heads.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] H. Nakajima et al.: "Scanning Force Microscopy of the Interaction Events between a Single Molecule of Heavy Meromyosin and Action." Biochem. Biophys. Res. Commun.234. 178-182 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Kunioka & T. Ando: "Innocuous Labeling of the Subfragment-2 Region of Skeletal Muscle Heavy Meromyosin with a Fluorescent Polyacrylamide Nanobead and Visualization of Individual Heavy" J. Biochem.119. 1024-1032 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤 敏夫: "原子力間顕微鏡とその応用" 細胞工学. 17. 458-468 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤 敏夫 他14名: "生体分子モーターの仕組み" 共立出版, 214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nakajima et al.: ""Scanning Force Microscopy of the Interaction Events between a Single Molecule of Heavy Meromyosin and Actin."" Binochem.Biophys.Res.Commun. 234. 178-182 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Kunioka & T.Ando: ""Innocuous Labeling of the Subfragment-2 Region of Skeletal Muscle Heavy Meromyosin with a Fluorescent Polyacrylamide Nanobead and Visualization of Individual Heavy Meromyosin Molecules"" J.Biochem. 119. 1024-1932 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ando: ""Atomic Force Microscope and its Applications"" Cell Engineering. 17. 458-468 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ishiwata et al.: "Mechanisms of Biological Molecular Motors". Kyoritsu Press, Inc., 214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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