1996 Fiscal Year Final Research Report Summary
Immortalization of multipotent stem cells by using the oncogene transgenic mice
| Project/Area Number |
06558104
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Cell biology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
OBINATA Masuo Institute of Development, TOHOKU UNIVERSITY,Aging and Cancer, Professor, 加齢医学研究所, 教授 (10099971)
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| Co-Investigator(Kenkyū-buntansha) |
HORIUCHI Tadashi Daiichi Pharmaceutical Co., Ltd., Basic Technology Research Laboratory,, 創薬基盤研究所, 所長
MATSUI Yasuhisa Institute of Development, TOHOKU UNIVERSITY,Aging and Cancer, Associate Professo, 加齢医学研究所, 助教授 (40241575)
YANAI Nobuaki Institute of Development, TOHOKU UNIVERSITY,Aging and Cancer, Assistant Professo, 加齢医学研究所, 助手 (80200525)
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| Project Period (FY) |
1994 – 1996
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| Keywords | multipotent stem cell / immortalization / transgenic mice / hematopoietic stem cell / gastric mucous cell / primordial germ cell |
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| Research Abstract |
To establish a variety of immortalized multipotent stem cell lines, the transgenic mice harboring temperature-sensitive T-antigen (ts-antigen) gene of simian virus 40 (SV40) were used.
To establish multipotent hematopoietic stem cell lines, the sorted stem cells were cocultured with the bone marrow stromal cell lines and two immature hematopoietic stem-like cell lines were immortalized depending on thr stromal cells. One cell line is a B-lymphoid stem cell line and self-renewed as well as committed to differentiate more mature B cells. The other cell lines were obtained from the transgenic mice and exhibited surface markers of the stem cells and can be differentiated to more mature hematopoietic cells.
Several transgenic mice with bcl-2 gene, c-kit gene, or Sl gene were made and used to immortalize primordial germ cells, and the germ cells from such transgenic mice showed their prolonged maintenance in culture, but the immortalization was not yet observed.
Gastric mucosal cells were also established fro the transgenic mice and showed specific functions of normal gastric mucosal cells including production of protective factors such as glycoproteins, bicarbonate, and prostaglandins.
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[Publications] Mataga, N., Tamura, M., Yanai, N., Shimomura, T., Kimata, K., Obinata, M.and Noda, M.: "Establishment of a novel chondrocyte-like cell line derived from transgenic mice harboring temperature sensitive Simian Virus 40 large T-antigen gene." J.Bone and Mineral Res.11. 1646-1654 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kodaira, K., Takahashi, R., Hirabayashi, M., Suzuki, T., Obinata, M.and Ueda, M.: "Overexpression of c-Myc induces apoptosis at prophase of rat primary spermatocytes." Mol.Reprod.Dev.45. 403-410 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsubara, N., Takahashi, Y., Nishina, Y., Mukouyama, Y., Yanagisawa, M., Watanabe, T., Nakano, T., Nomura, K., Arita, H., Nishimune, Y., Obinata, M.and Matsui, Y.: "A receptor tyrosine kinase, Sky, and its ligand Gas 6 are expressed in gonads and support primodial germ cell growth or survival in culture." Develop.Biol.180. 499-510 (1996)
Description
「研究成果報告書概要(欧文)」より
