1996 Fiscal Year Final Research Report Summary
Establishment of serodiagnostic method and surveylance system for hemorrhagic fever with renal syndrome virus infection in experimental animals
| Project/Area Number |
06558112
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Laboratory animal science
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARIKAWA Jiro Hokkaido University School of Medicine, Institute for Animal Experimentation, Professor, 医学部, 教授 (10142704)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Akira Division of Microbiology ICLAS Monitoring Center, Central Laboratory of Experime, 微生物モニタリングセンター, 室長代理 (60167484)
SUGIYAMA Kazuyoshi Section of Biosafety, National Institute of Health, Japan, Chief, ウイルスー部, 室長 (90150185)
YOSHIMATSU Kumiko Hokkaido University School of Medicine, Institute for Animal Experimentation, As, 医学部, 助手 (90220722)
KARIWA Hiroaki Graduate School of Veterinary Medicine, Hokkaido University, Lecturer, 大学院・獣医学研究科, 講師 (70224714)
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| Project Period (FY) |
1994 – 1996
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| Keywords | HFRS / Hantavirus / Baculovirus / Serological diagnosis / ELISA / Westem blotting / Experimental animals / Zoonosis |
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| Research Abstract |
1. Recombinant baculovirus expressing hantavirus nucleocapsid protein (Bac.HTN-NP) was applied for the antigen of ELISA.Specificity of the ELISA was the same to that of the IFA which uses infected Vero E6 cells as antigen. However, the ELISA had a sensitivity problem because of the relatively high background coloring.
2. cDNA which encoded hantavirus nucleocapsid protein was expressed by using E.coli system and established capture ELISA system. This ELISA was shown to have same specificity and sensitivity to that of the ordinally IFA,and therefore, considered as a useful screening method.
3. Bac.HTN-NP was successfully applied for the antigen for Western blotting (WB). The WB method readily distinguished specific reaction from nonspecific reaction from the WB pattern. Therefore, the WB was considered as an effective method for the serologic confirmation of hantavirus infection.
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Research Products
(14 results)
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[Publications] Yoshimatsu, K., Arikawa, J., Yoshida, R., Li, H., Yoo, Y-C., Kariwa, H., Hashimoto, N.and Azuma, I.: "Production and application of recombinant hantavirus nucleocapsid protein expressed in silkworm larvae as a diagnostic antigen for laboratory rat." Lab.Anim.Sci.45. 647-651 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kariwa, H., Yoshizumi, S., Arikawa, J., Yoshimatsu, K., Takahashi, K., Takashima, I., Hashimoto, N: "Evidence for the existence of Puumalarelated virus Clethrionomys rufocanus in Hokkido, Japan." Amer.J.Trop.Med.Hyg.53. 222-227 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kariwa, H., Kamimura, M., Arikawa, J., Yoshimatsu, K., Takahashi, K., Takashima, I., Hashimoto, N.: "Characterization of the mode of Hantaan virus infection in adults mice using a nested reverse transcriptase polymerase chain reaction : transient virus replication in adult mice." Microbiol.Immunol.39. 35-41 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoo, Y-C., Yoshimatsu, K., Hatsuse, R., Yoshida, R., Tonooka, S., Arikawa, J., Azuma, I.: "Effect of MDP-Lys (L18), a derivatives of MDP,on enhancing host resistance against Hantaan virus infection in newborn mice." Vaccine. 13. 1300-1305 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshimatsu, K., Arikawa, J., Li, H., Kariwa, H., Hashimoto, N.: "Western blotting using recombinant Hantaan Virus nucleocapsid protein expressed in silkworm as a serological confirmation of hantavirus infection in human sera.J.Vet.Med.Sci.58 : 71-74,1996" J.Vet.Med.Sci.58. 71-74 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshimatsu, K., Arikawa, J., Tamura, M., Yoshida, R., Lundkvist, A., Niklasson, B., Kariwa, H., Azuma, I.: "Characterization of the nucleocapsid protein of hantaan virus strain 76-118 using monoclonal antibodies." J.Gen.Virol.77. 695-704 (1996)
Description
「研究成果報告書概要(欧文)」より
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