1994 Fiscal Year Annual Research Report

TED遺伝子群を用いた維管束分化の解析

Research Project

Project/Area Number	06640831
Research Institution	Tohoku University
Principal Investigator	福田裕穂東北大学, 理学部, 教授 (10165293)
Keywords	ヒャクニチソウ / 細胞分化 / 維管束 / 管状要素 / cis因子 / アンチセンスDNA / 遺伝子導入 / TED
Research Abstract	ヒャクニチソウの単離葉肉細胞から管状要素への分化過程で、形態形成に先立ち分化に特異的に発現する遺伝子TED2,TED3,TED4をマーカーとして用いて、維管束分化の仕組みについて解析することを目的とした。まず、TED3のゲノムDNAの断片をGUS遺伝子につなぎシロイヌナズナに導入し、形質転換個体を得た。得られた形質転換個体を解析した結果、TED3ゲノムの転写開始点から上流約500bpのうちに、木部細胞特異的に発現する調節領域が存在することが明らかになった。この領域にはこれまで報告されている木部特異的な促進cis因子は見られず、これまでにないcis因子が存在することが予想された。次に、TED2タンパク質の発現・機能を知るために、まず、TED2の遺伝子産物を大腸菌中で発現させ、これを用いて抗体を作成した。この抗体を用いて、in vitroの管状要素分化過程および、個体内での維管束発達過程でのタンパク質の発現を調べたところ、mRNAとほぼ同様な発現パターンを示し、TED2タンパク質の発現は、主に転写レベルで制御されていることが明らかになった。さらに、TED2の機能を解析するために、ヒャクニチソウ形質転換根系を用いて、TED2遺伝子のアンチセンスDNAの遺伝子導入を行った。その結果、TED2のアンチセンスDNAを導入した形質転換根の中に、TED2タンパク質の発現が抑えられると同時に、維管束の異常が見られるものがあり、TED2タンパク質は維管束形成において重要な役割を果たしている可能性が示唆された。

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Fukuda,H.: "Redifferentiation of single mesophyll cells into tracheary elementsentiation" International Journal of Plant Science. 155. 262-271 (1994)
[Publications] Sugiyama,M.: "Characteristics of the inhibitory effects of aphidicolin on transdifferentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans." Plant Cell Physiology. 35. 519-522 (1994)
[Publications] Demura,T.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development" Plant Cell. 6. 967-981 (1994)
[Publications] Ito,M.: "Meristem-specific gene expression directed by the promoter of the S-phase-specific gene,cyc07" Plant Molecular Biology. 24. 863-878 (1994)
[Publications] Kobayashi,H.: "Involvement of Calmodulin and Calmodulin-binding proteins in the differentiation of tracheary elements in Zinnia cells" Planta. 194. 388-394 (1994)
[Publications] Kawahara,R.: "Analysis by in situ hybridization of the expression of elongation factor 1a in the carrot cells during somatic embryogenesis" Journal of Plant Research. 107. 361-364 (1994)
[Publications] Fukuda,H.: "Mechanisms of the proliferation and differentiation of plant cells in cell culture systems" International Journal of Developmental Biology. 38. 287-299 (1994)
[Publications] Sato,Y.: "Separation and characterization of the isozymes of wall-bound peroxidase from cultured Zinnia cells during tracheary element differentiation" Planta. (in press). (1994)
[Publications] Kawahara,R.: "Isolation and characterization of homeobox-containing genes of carrot" Plant Molecular Biology. (in press). (1995)
[Publications] Sugiyama,M.: "Zinnia mesophyll culture system to study xylogenesis" Plant Tissue Culture Manual,Kluwer Academic Press. (in press). (1995)
[Publications] Joy,R.W.IV: "Cloning and characterization of polyphenol oxidase cDNAs of Phytolacca americana" Plant Physiology. (in press). (1995)

1994 Fiscal Year Annual Research Report

TED遺伝子群を用いた維管束分化の解析

Principal Investigator

福田 裕穂 東北大学, 理学部, 教授 (10165293)

Research Products

[Publications] Fukuda,H.: "Redifferentiation of single mesophyll cells into tracheary elementsentiation" International Journal of Plant Science. 155. 262-271 (1994)

[Publications] Sugiyama,M.: "Characteristics of the inhibitory effects of aphidicolin on transdifferentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans." Plant Cell Physiology. 35. 519-522 (1994)

[Publications] Demura,T.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development" Plant Cell. 6. 967-981 (1994)

[Publications] Ito,M.: "Meristem-specific gene expression directed by the promoter of the S-phase-specific gene,cyc07" Plant Molecular Biology. 24. 863-878 (1994)

[Publications] Kobayashi,H.: "Involvement of Calmodulin and Calmodulin-binding proteins in the differentiation of tracheary elements in Zinnia cells" Planta. 194. 388-394 (1994)

[Publications] Kawahara,R.: "Analysis by in situ hybridization of the expression of elongation factor 1a in the carrot cells during somatic embryogenesis" Journal of Plant Research. 107. 361-364 (1994)

[Publications] Fukuda,H.: "Mechanisms of the proliferation and differentiation of plant cells in cell culture systems" International Journal of Developmental Biology. 38. 287-299 (1994)

[Publications] Sato,Y.: "Separation and characterization of the isozymes of wall-bound peroxidase from cultured Zinnia cells during tracheary element differentiation" Planta. (in press). (1994)

[Publications] Kawahara,R.: "Isolation and characterization of homeobox-containing genes of carrot" Plant Molecular Biology. (in press). (1995)

[Publications] Sugiyama,M.: "Zinnia mesophyll culture system to study xylogenesis" Plant Tissue Culture Manual,Kluwer Academic Press. (in press). (1995)

[Publications] Joy,R.W.IV: "Cloning and characterization of polyphenol oxidase cDNAs of Phytolacca americana" Plant Physiology. (in press). (1995)

福田裕穂東北大学, 理学部, 教授 (10165293)