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1995 Fiscal Year Final Research Report Summary

Cellular basis for chromosome behavior and microtubule organization in meiosis.

Research Project

Project/Area Number 06640868
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物形態・構造
Research InstitutionYokohama City University

Principal Investigator

ANAKA Ichiro  Yokohama City University Department of Biology Associate Professor, 理学部, 助教授 (60175445)

Project Period (FY) 1994 – 1995
KeywordsMeiosis / Pollen mother cell / Embryosac mother cell / Microtubule / Univalent chromosome / Centromere / Spindle / Phragmoplast
Research Abstract

The dynamics of microtubule (MT) organization during normal and abnormal meiosis in microsporocytes and megasporocytes of Lilium was investigated with immuno fluorescence using CLSM (confocal laser scanning microscopy) and cryo-sections. MTs radiating from the nucleus at prophase I seemed to be integrated into the bipolar spindle after the nuclear envelope breakdown. On the other hand, phragmoplast MTs seemed not to function in cell plate formation. It was also supposed that the centromeres (kinetochores) play important roles in spindle formation during meiosis. Fortunately, we discovered an antiserum, which preferentially stains the centromere region of meiotic chromosomes in microsporocytes and megasporocytes of Lilium longiflorum, among antisera raised against a variant of histone H1 extracted from pachytene nuclei of this lily. Using this antiserum, we were able clearly to visualize the meiosis-specific dynamics of the centromere, namely, the association and fusion of homologous centromeres at zygotenepachytene in prophase I,the disjunction of the homologous centromeres at diplotene, the doubling of each centromere at metaphase I,non-separation of the sister centromeres at anaphase I and their separation at anaphase II by CLSM.By contrast, this antiserum did not recognize the centromere region of mitotic chromosomes in root-tip meristem. From these results, it is suggested that the antigen is a constitutive centromere protein required for meiosis-specific centromere functioncommon to male and female meiosis. Double staining with anti-tubulin antibody is now in progress to ascertain the relationship between the centromere structure and microtubule attachment.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ueda,K.: "Basic proteins of male gametic nuclei is olated from pollen grains of Lilium longiflorum." Planta. 192. 446-452 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中一朗: "花粉の発生" プランタ. 36. 26-31 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda,K.: "The appearance of male gamete-specific histones gH2B and gH3 during pollen development in Lilium longiflorum." Dev.Biol.169. 210-217 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鍋谷真範: "テッポウユリ花粉発生過程におけるアミロプラストの発達と花粉粒内分布" 日本花粉学会会誌. 41(1). 13-19 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyoshi,H.: "High level of GUS gene expression driven by pollen-specific promoters in electroporated lily pollen protoplasts." Sex.Plant Reprod.8. 205-209 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda,K.: "Male gametic nucleus-specific H2B and H3 histones,designated gH2B and gH3,in Lilium longiflorum." Planta. 197. 289-295 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日本花粉学会: "花粉学事典" 朝倉書店, 454 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jain,S.M.: "In Vitro Haploid Production in Higher Plants. Vol.2" Kluwer Academic Publishers, 438 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda, K.and I.Tanaka: "Basic proteins of male gametic nuclei isolated from pollen grains of Lilium longiflorum." Planta. 192. 446-452 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, K.and I.Tanaka: "The appearance of male gamete-specific histones gH2B and gH3 during pollen development in Lilium longiflorum." Dev.Biol.169. 210-217 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyoshi, H., Usami, T.and I.Tanaka: "High level of GUS gene expression driven by pollen-specific promoters in electroporated lily pollen protoplasts." Sex.Plant Reprod.8. 205-209 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, K.and I.Tanaka: "Male gametic nucleus-specific H2B and H3 histones, designated gH2B and gH3, in Lilium logiflorum." Planta. 197. 289-295 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jain, S.M., Sopory, S.K.and R.E.Veilleux: In Vitro Haploid Production in Higher Plants. Vol.2. Kluwer Academic Publishers, 438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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