1995 Fiscal Year Final Research Report Summary
Genome Mapping of Plant Pathogenic Bacteria
| Project/Area Number |
06660047
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Shizuoka University |
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Principal Investigator |
TAKIKAWA Yuichi Associate Professor, Faculty of Agriculture, Shizuoka University, 農学部, 助教授 (90163344)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Plant pathogenic bacteria / Pseudomonas / Gene library |
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| Research Abstract |
Construction of gene libraries from several Pseudomonas plant pathogens were challenged. During these two years, the libraries were successfully constructed from P.syringae pv.phaseolicola, P.s.glycinea, P.s.maculicola, P.s.tabaci, P.s.syringae, and P.s.actinidiae. From the libraries, the clones which have homology with hrp genes and phaseolotoxin production genes were selected by hybridization and genomic walking. As for hrpregions, a map was obtained on a long area covering more than 120kb. The homologous regions in the clones from different bacteria are preserved for more than several 10kb in length in each directions beyond the known hrp region. The homologies sharply declined toward the out side of the region. As for phaseolotoxin production genes, clones were obtained from P.s.glycinea which showed homology with the borders of the phaseolotoxin production genes. The clones were aligned and mapped. The result showed that the borders from P.s.glycinea are intervened by a 12kb unknown fragment which has no homology with phaseolotoxin production genes. That strongly suggested that the phaseolotoxin production genes have moved horizontally among bacteria. Unfortunately, entire mapping using puls-field electrophoresis and PCR was unsuccessful, though, the results obtained were valuable indicating that the strategy of genome mapping can lead to the elucidation of the evolutionary process in plant pathogenic bacteria.
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