1995 Fiscal Year Final Research Report Summary
Translational control by the phosphorylation of elongation factor 1
| Project/Area Number |
06660085
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Iwate University |
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Principal Investigator |
EJIRI Shin-ichiro Iwate University, Department of Agriculture, Professor, 農学部, 教授 (90005629)
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| Project Period (FY) |
1994 – 1995
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| Keywords | peptide chain elongation factor / phosphorylation / EF-1 |
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| Research Abstract |
Phosphorylation has been established as a major mechanism by which metabolic processes are regulated. Many factors concerning protein biosynthesis are regulated by phosphorylation and dephosphorylation : aminoacyl-tRNA synthetase, initiation factors, elongation factors EF-1 and EF-2, and ribosomal proteins are regulated by various protein kinases. In the present research the mechanism of translational control by the phosphorylation of elongation factor EF-1 beta has been analyzed.
Casein kinase-like EF-1 beta kinase which phosphorylates beta subunit of EF-1 (EF-1 alpha beta beta'gamma) was purified from wheat embryo. By the phosphorylation, EF-1 beta activity (GDP/GTP exchange activity) was stimulated. In contrast to these results, animal EF-1 delta which corresponds to plant EF-1 beta was inhibited by the phosphorylation. Interestingly, the phosphorylation sites on EF-1 beta and EF-1 delta are different.
In the course of these experiments, "simple method for analyzing phosphoamino acid by thin-layr chromatography" had been established, and the method was applied for the analysis of the phosphorylation of EF-1 from wheat embryo.
In the present research, "continuously coupled transcription-translation system for the production of rice cytoplasmic aldolase" had been established also.
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Research Products
(4 results)
