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1995 Fiscal Year Final Research Report Summary

Delineation of DNA Replication Timing within MHC Region by FISH

Research Project

Project/Area Number 06660099
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionMie University

Principal Investigator

OKURMURA Katsuzumi  Mie Univ.Fac.Bioresources, Assoc.Prof., 生物資源学部, 助教授 (30177183)

Project Period (FY) 1994 – 1995
KeywordsFISH / MHC / DNA replication / chromosome structure / GC content
Research Abstract

Fluorescence in situ hybridization (FISH) was used to analyze how the DNA replication timing is related to the genome structure and how the specific genome domains are arranged in the nucleus.
First, we have determined the replication timing pattern of DNA segments over 2Mb within human MHC on HL60 cells. This region is composed of long-range G+C% (GC) mosaic structures related to chromosome bands and a boundary of GC mosaic domains exists between MHC classes II and III.FISH with the elongated chromatin fibers showed that the flanking genome structures of the boundary are strictly consistent with the reported mapping data. This confirmed that HL60 cells may not have large structural abnormality in this region. Multi-color FISH to interphase nuclei was useful to compare the temporal replication timing of two DNA fragments which locate adjacent genomic positions and have a similar replication timing in the cell cycle. By this procedure, it was possible to determine the precise replication timing of each DNA fragment which was randomly selected from MHC region. The fragments at the boundary region were found to relatively replicate latest among all examined. It was also suggested that this region consists of at least two replicon clusters. Interestingly, there is a close correlation between the temporal order of replication and the GC gradient of genome sequences in human MHC on HL60 cells. These results suggested the possibility that replication of DNA in mammalian cell genome reflects GC mosaic domains at the level of genome sequences as well as chromosome bands.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kaisuzumi Okumura: "The Genes for the α-Type HC3 (PSMA2) and β-Type HC5 (PSMBI) Subunits of Human Proteasomes Map to Chromosomes 6q27 and 7p12-p13 by Fluorescence inSitu Hybridization."

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Genomics. 27. 377-379 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akiko Shiratori: "Assignmnt of the 49-kDa(RPIM1)and 58-kDa(PRIM2A and PRIM2B) Subunit Genes of the Human DNA Primase to Chromosome Bands 1q44 and 6p11,1-p12." Genomics. 28. 350-353 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Klyoyuki Torlgoe: "YAC-based 1.5 Mb Contig Spanning the Human Multidrug Resistance Gene Region and Delineating the Amplification Unit in Two Human Multidrug Resistant Cell Lines." Genome Research. 5. 233-244 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitoshi Kusaba: "Functional Expression of Yeast Artificial Chromosome (YAC)-Human Multidrug Resistance Genes in Mouse Cells." Genome Research. 5. 245-258 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatso Fukagawa: "Human Pseudoautosomal Boundary-Like Sequences (PABLs) : Expression and a Model of Formation of Present-day Pseudoautosomal Boundary of Sex Chromosomes." Human Molecular Genetics. 5. 23-32 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuhisa Mizuki: "Isolation of cDNA and Genomic Clones for a Human Ras-related GTP-Binding Protein and lts Chromosomal Localization to the Long Arm of Chromosome 7,7q36" Genomics. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuzumi Okumura, et al: "The Genes for the alpha-Type HC3 (PSMA2) and beta-Type HC5 (PSMB1) Subunits of Human Proteasomes Map to Chromosomes 6q27 and 7p12-p13 by Fluorescence in Situ Hybridization :" Genomics. 27. 377-379 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akiko Shiratori, et al: "Assignment of the 49-kDa (RPIM1) and 58-kDa (PRIM2A and PRIM2B) Subunit Genes of the Human DNA Primase to Chromosome Bands 1q44 and 6p11.1-p12 :" Genomics. 28. 350-353 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoyuki Torigoe, et al: "YAC-based 1.5Mb Contig Spanning the Human Multidrug Resistance Gene Region and Delineating the Amplification Unit in Two Human Multidrug Resistant Cell Lines :" Genome research. 5. 233-244 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitoshi Kusaba, et al: "Functional Expression of Yeast Artificial Chromosome (YAC)-Human Multidrug Resistance Genes in Mouse Cells :" Genome Research. 5. 245-258 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsuo Fukagawa, et al: "Human Pseudoautosomal Boundary-Like Sequences (PABLs) : Expression and a Model of Formation of Present-day Pseudoautosomal Boundary of Sex Chromosomes :" Hum.Mol.Genet.5(1). 23-32 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuhisa Mizuki, et al: "Isolation of cDNA and Genomic Clones for a Human Ras-related GTP-Binding Protein and Its Chromosomal Localization to the Long Arm of Chromosome 7,7q36 :" Genomics. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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