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1995 Fiscal Year Final Research Report Summary

The study of signal transduction mechanism of angiotensin II receptor by mean of site-directed mutagenesis

Research Project

Project/Area Number 06660107
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionTottori University

Principal Investigator

YAMANO Yoshiaki  Tottori University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (00182593)

Co-Investigator(Kenkyū-buntansha) MORISHIMA Isao  Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (30032296)
Project Period (FY) 1994 – 1995
KeywordsAngiotensin II / Receptor / Site-directed mutagenesis / cDNA
Research Abstract

Ligand binding of rat angiotensin II receptor (AT1A)
The molecular interaction involved in the ligand binding of rat angiotensin II receptor (AT1A) was studied by site-directed mutagenesis and receptor model building. Five conserved residues in AT1A,R167, K199, W253, F259 and D263 were substituted with alanine, individually. These amino acid residues appeared to provide binding sites for angiotensin II.Binding of a nonpeptide angiotensin II antagonist was also reduced for R167 mutant. R167 is concerned for receptor conformation.
G-protein coupling of rat angiotensin II receptor (AT1A)
G-protein coupling in carboxyl-terminal tail of rat angiotensin II receptor was studied by site-directed mutagenesis. Deletion mutants, which have stop codons at K310 (K310DELTA) or at K318 (K318DELTA), have angiotensin II binding affinity similar to wild type receptor. In case of K318DELTA, both the effects of GTPgammaS and the IP3 production was retained in response to angiotensin II,but K310DELTA had neither of the effects. It is concluded that G-protein couples to amino acid residues between K310 and K318.
Expression of the chicken angiotensin II receptor in adrenal
Aves have a unique angiotensin II receptor, whose character is different from AT1 and AT2. The probe used for the hybridization to determine the expression of the receptor was synthesized using PCR.The receptor was expressed in adrenal but not in liver.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Yoshiaki Yamano: "Mutagenesis and the molecular modeling of the rat angiotensin II receptor (AT_1)." J. Biol. Chem.270. 14024-14030 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Ohyama: "Disulfide bridgens in extracellular domains of angiotensin II receptor type I-A." Regulatory peptides. 57. 141-147 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoshi Mikawa: "Dynamic aspects in the expression of the goat insulin-like growth factor-I (IGF-I) gene: diversity in transcription and post-transcripotion." Biosci. Biotech. Biochem.59. 87-92 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoshi Mikawa: "Tissue-and development-specific expression of goat insulin-like growth factor-I (IGF-I) mRNAs." Biosci. Biotech. Biochem.59. 759-761 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiaki Yamano: "Cloning of cDNA for cecropin A and B, and expression of the genes in the silkworm, Bombyx mori" Biosci. Biotech. Biochem. 58. 1476-1478 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeyuki Chaki: "Role of carboxyl tail of the rat angiotensin II type IA receptor in agonist-induced internalization of the receptor" Kidney International. 46. 1492-1495 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 駒野 徹: "生物化学実験法 33 組換えDNA実験入門" 学会出版センター, 237 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yamano, K.Ohyama, M.Kikyo, T.Sano, Y.Nakagomi, Y.Inoue, N.Nakamura, I.Morishima, D.-F.Guo, T.Hamakubo and T.Inagami: "Mutagenesis and the molecular modeling of the rat angiotensin II receptor (AT1)." J.Biol.Chem.270. 14024-14030 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ohyama, Y.Yamano, T.Sano, Y.Nakagomi, T.Hamakubo, I.Morishima and T.Inagami: "Disulfide bridges in extracellular domains of angiotensin II receptor type 1-A." Regulatory Peptides. 57. 141-147 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mikawa, G.Yoshikawa, H.Aoki, Y.Yamano, H.Sakai and T.Komano: "Dynamic aspects in the expression of the goat insulin-like growth factor-I (IGF-I) gene : diversity in transcription and post-transcription." Biosci.Biotech.Biochem.59. 87-92 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mikawa, G.Yoshikawa, Y.Yamano, H.Sakai, T.Komano, Y.Hosoi and K.Utsumi: "Tissue-and development-specific expression of goat insulin-like growth factor-I (IGF-I) mRNAs." Biosci.Biotech.Biochem.59. 759-761 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Yamano, M.Matsumoto, K.Inoue, T.Kawabata and I.Morishima: "Cloning of cDNA for cecropin A and B,and expression of the genes in the silkworm, Bombyx mori." Biosci.Biotech.Biochem.58. 1476-1478 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Chaki, D.-F.Guo, Y.Yamano, K.Ohyama, M.Tani, M.Mizukoshi, H.Shirai and T.Inagami: "Role of carboxyl tail of the rat angiotensin II type IA receptor in agonist-induced internalization of the receptor." Kidney International. 46. 1492-1495 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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