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1996 Fiscal Year Final Research Report Summary

Elucidation of Functional Implication of Post-translational Tyrosine Sulfation

Research Project

Project/Area Number 06660117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionMiyazaki University

Principal Investigator

SUIKO Masahito  Miyazaki University, Department of Biological Resources Sciences, Associate Professor, 農学部, 助教授 (00128357)

Project Period (FY) 1994 – 1996
KeywordsTyrosine Sulfation / Post-translational Modification / Sulfotransferase / Golgi Body / Sorting / Microsome / Complement C3 / Fibronectin
Research Abstract

By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver TyrS-binding protein in complexes with tyrosine-sulfated proteins both in vivo and in vitro, suggesting the putative role of the former being the receptor for the latter. Using filter-grown Madin-Darby canine kidney (MDCK) cells as a model, it was demonstrated that the tyrosine-sulfated proteins synthesized were predominantly secreted into the apical medium. The results further indicate the production and differential polarized secretion of different sulfated forms of the two major secretory proteins produced by MDCK cells, fibronectin (FN) and an 80 kDa glycoprotein (gp 80), with their tyrosine-sulfated forms being predominantly secreted from the apical surface. Treatment of filtergrown MDCK cells with glycosylation inhibitors, swainsonine and 1-deoxymannojirimycin, appeared to enhance the apical secretion of tyrosine-sulfated FN and gp 80. A similar 175 kDa membrane-bound "TyrS trceptor." cross-reactive toward antiserum against the canine liver TyrS receptor. was shown to be present in MDCK cells. Pulse-chase experiments revealed its presence in complexes with newly synthesized FN and gp 80. A hypothetical model for TyrS residues serving as an apical targeting signal during the biosynthetic transport of tyrosine-sulfated proteins. as mediated by the TyrS receptor. in MDCK cells is proposed.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 水光 正仁: "遊離チロシンの硫酸化の意義:ドーパ、カテコールアミンなどの体内濃度調節に関与か?" 化学と生物. 34(7). 422-424 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Liu,Ming-Cheh: "Biochemistry of the Sulfation of Dopa and Tyrosine Isomers:Investigation of a Novel Dopa/Tyrosine Sulfotransferase." Cytotechnology. 1-7 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoichi Sakakibara: "Role of a putaive tyrosine-O-sulfate receptor in the targeting and/or intracellular trnsport of tyrosine-sulfated proteins." Animal Cell Technology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoichi Sakakibara: "Stereoselective and Manganese-Dependent Dopa/Tyrosine Sulfation in HepG2 Human Hepatoma Cells:Association of the Dopa/Tyrosine Sulfotransferase Activities with the Human″Monoamine-Form″Phenol Sulfotransferase." Biochim.Biophys.Acta. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoichi Araki: "Tissue-specific and developmental stage-dependent expression of a novel rat Dopa/tyrosine sulfotransferase." Intl.J.Biochem.Cell Biology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 水光 正仁: "タンパク質化学 酵素4.2トランスフェラーゼ(硫酸転移酵素)" 廣川書店(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SUIKO,MASAHITO: "Functional Implication of Tyrosine Sulfation" Chemistry and Biology. 34(7). 422-424 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] LIU,MING-CHEH: "Role of a putative tyrosine-O-sulfate receptor in the targeting and/or intracellular transport of tyrosine-sulfated proteins." Cytotechnology. 1-7 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SAKAKIBARA,YOICHI: "Biochemistry of the Sulfation of Dopa and Tytosine Isomers : Investigation of a Novel Dopa/Tyrosine Sulfotransferase." Animal Cell Technology. Vol.7, Kluwer Academic Publishers, (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SAKAKIBARA,YOICHI: "Stereoselective and Manganese-Dependent Dopa/Tyrosine Sulfation in HepG2 Human Hepatoma Cells : Association of the Dopa/Tyrosine Sulfotransferase Activities with the Human "Monoamine-Form" Phenol Sulfotransferase." Biochim.Biophys.Acta. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ARAKI,YOICHI: "Tissue-specific and developmental stage-dependent expression of a novel rat Dopa/tyrosine sulfotransferase." Intl.J.Biochem.Cell Biology. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SUIKO,MASAHITO: Transferase : Sulfotransferase, Protein Chemistry. Hirokawa Shoten (in press), (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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