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1996 Fiscal Year Final Research Report Summary

Isolation of centromeric DNA by utilizing the DNA binding activity of recombinant centromere proteins.

Research Project

Project/Area Number 06660120
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionUniversity of Osaka Prefecture

Principal Investigator

SUGIMOTO Kenji  University of Osaka Prefecture, College of Agriculture, Assistant Professor, 農学部, 講師 (40196746)

Project Period (FY) 1994 – 1996
Keywordscentromere protein / DNA binding / centromere DNA
Research Abstract

Centromere/kinetochore is defined as the microtubule attachment site at the primary constriction of chromosomes. Electron microscopy studies have revealed that mammalian kinetochore has the trilaminar structure. However, the molecular basis of centromere/kinetochore structure has not been characterized extensively. It is assembled from the underlying centromere heterochromatin during the cell division cycle. Thus far, three major centromere autoantigens (CENtromere proteins ; CENP-A,CENP-B and CENP-C) together with HP1HSalpha, human homolog of Drosophila heterochromatin-associated protein 1, have been shown to be localized to centromere throughout the cell cycle. We have shown that they are structural components of centromere/kinetochre heterochromatin which possess DNA-binding activity in vitro and determined the DNA-binding domains. In this project, we further characterize the molecular interactions between DNA and CENPs further and identify novel centromere/kinetochore proteins that are associated with these CENPs in interphase and metaphase chromosomes by molecular biological and biochemical procedures.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] K.Sugimoto, Y.Hagishita and M.Himeno: "Functional domain strucuture of human centromere protein B: Implication of the internal and C-terminal self-association domains in centromeric heterochromatin condensation." Journal of Biological Chemistry. 269. 24271-24276 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugimoto, K.Furukawa, and M.Himeno: "Functional cloning of centromere protein B(CENP-B) box-entiched alphoid DNA repeats utilizing the sequence-specific DNA binding activity of human CENP-B in vitro." Chromosome Research. 2. 453-459 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugimoto, H.Yata, Y.Muro, and M.Himeno: "Human centromere protein C (CENP-C) is a DNA-binding protein which possesses a novel DNA-binding motif." Journal of Biochemistry. 116. 877-881 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugimoto, T.Yamada, Y.Muro, and M.Himeno: "Human homolog of Drosophila heterochromatin-associated protein 1 (HP1) is a DNA-binding protein which possesses a DNA-binding motif with weak similarity to that of human centromere protein C (CENP-C)." Journal of Biochemistry. 120. 153-159 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Sugimoto: "DNA-protein interaction involved in centromere heteochromatin organization of human chromosome." Viva Origino. 24. 211-224 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugimoto, K.Kuriyama, A.Shibata, and M.Himeno: "Characetrization of internal DNA-binding and C-terminal dimerization domains of human centromere/Kinetochore autoantigen CENP-C in vitro: Role of DNA-binding and self-associating activities in kinetochore organization." Chromosome Research. 5. 1-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Sugimoto: "DNA immunoprecipitation: Application to characterization of terget sequences for a human centromere DNA-binding protein (CENP-B) in "Cell Biology: A Laboratory Handbook"" J.E.Celis, Academic Press, 9 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugimoto, Y.Muro, M.Ohashi, and M.Himeno: "Scleroderma diagnosis with recombinant protein. in "Encyclopedia of Molecular Biology and Molecular Medicine"." R.A.Meyers,VCH Publishers, 11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugimoto, Y.Hagishita, and M.Himeno.Functional domain strucuture of human centromere protein B: "Implication of the internal and C-terminal self-association domains in centromeric hetero-chromatin condensation." J.Biol.Chem.269. 24271-24276 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto, K.Furukawa, M.Himeno.: "Functional cloning of centromere protein B (CENP-B) box-enriched alphoid DNA repeats utilizing the sequence-apecific DNA binding activity of human CENP-B in vitro." Chromosome Res.2. 453-459 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto, H.Yato, Y.Muro, and M.Himeno.: "Human centromere protein C (CENP-C) is a DNA-binding protein which possesses a novel DNA-binding motif." J.Biochem.116. 877-881 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto, T.Yamada, Y.Muro, and M.Himeno.: "Human homolog of Drosophila heterochromatin-associated protein 1 (HP1) is a DNA-binding protein which possesses a DNA-binding motif with weak similarity to that of human centromere protein C (CENP-C)." J.Biochem.120. 153-159 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto: "DNA-protein interaction involved in centromere heteochromatin organization of human chromosome." Viva Origino.24. 211-224 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto, K.Kuriyama, A.Shibata, and M.Himeno: "Characetrization of internal DNA-binding and C-terminal dimerization domains of human centromere/kinetochore autoantigen CENP-C in vitro : Role of DNA-binding and self-associating activities in kinetochore organization." Chromosome, Res.5. 1-10 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto: DNA immunoprecipitation : Application to characterization of target sequences for a human centromere DNA-binding protein (CENP-B). "Cell Biology : A Laboratory Handbook" ed by J.E.Celis.Academic Press, 335-343 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Muro, K.Sugimoto, M.Ohashi, and M.Himeno: Scleroderma diagnosis with recombinant protein. "Molecular Biology and Biotechnology" ed by R.A.Meyers. VCH Publishers, 853-856 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sugimoto, Y.Muro, M.Ohashi, and M.Himeno: Scleroderma diagnosis with recombinant protein. "Encyclopedia of Molecular Biology and Molecular Medicine".ed.by R.A.Meyers. VCH Publishers, 410-419 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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