1995 Fiscal Year Final Research Report Summary
Studies on the inhibitory mechanism of MATS,a garlic oil component, to the thromboxane synthesis by platelets.
| Project/Area Number |
06660165
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Nihon University |
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Principal Investigator |
ARIGA Toyohiko Nihon Univ., Agric.Vet.Med., Professor, 農獣医学部, 教授 (50096757)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Kenji Nihon Univ., Agric.Vet.Med., Professor, 農獣医学部, 教授 (80058776)
SAKURAI Hidetoshi Nihon Univ., Agric.Vet.Med., Assoc.Prof., 農獣医学部, 助教授 (80059617)
KUMAGAI Hitomi Nihon Univ., Agric.Vet.Med., Lecturer, 農獣医学部, 専任講師 (20225220)
SEKI Taiichiro Nihon Univ., Agric.Vet.Med., Lecturer, 農獣医学部, 専任講師 (20187834)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Garlic / Platelet / Arachidonic acid / Prostaglandin / Thromboxane / Cyclooxygenase / Aspirin / Endothelial cells |
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| Research Abstract |
A potent platelet aggregation inhibitor, methyl allyl trisulfide (MATS) found in the steam distillled oil components from garlic (Allium sativum L.) was studied to reveal its inhibition mechanism. MATS suppressed synthesis of thromboxane A_2 (TXA_2) in platelets, and showed quite different mode of inhibition from the most of all known specific inhibitors, e.g., aspirin for cyclooxygenase (COX) , esculetin for lipoxygenase (LPX) and OKY-046 for thrombaxane synthase (TXS). MATS inhibited both COX and LPX,but TXS at the concentration lower 10^<-4>M.MATS was judged to inactivate prostaglandin endoperoxide synthase (PGH synthase) , however, the inhibition was confined to the direction toward one of its activities, peroxidase activity. COX activity the another activity of this bifunctional enzyme, which has been found to be inhibited specifically by aspirin, seemed to be unaffected by MATS,since arachidonic acid was consumed with the metabolic rate similar to the control. Although, the detailed interaction between MATS and PGH synthase is not demonstrated yet, MATS would prevent the reduction of a substrate (PGG_2) through its alkyl sulfides structure. Against PGI_2 generation by the endothelial cells of aortic vessel walls, MATS also affected to reduce its levels. These results made us suspicious whether MATS acts practically antithrombotic. Then, we are about to investigate in vivo effect of MATS using an animal model of thrombosis.
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[Publications] Kumagai, H., Kashima, N., Seki, T., Sakurai, H., Ishii, K and Ariga, T.: "Analysis of volatile components in essential oil of upland wasabi and their inhibitory effects on platlet aggreaggation" Biosci.Biotech.Biochem.58(12). 2131-2135 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Ariga, T., Seki, T., Ando, K., Teramoto, S.and Nishimura, H.: Developments in Food Engineering. Yano.T., et al. (eds) (Anti-platelet principle found in the essential oil of garlic (Allium sativum L.) and its inhibition mechanism.). Blakie Academic Glasgow, NZ,& Prof., 1056-1058 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Ariga, T., Takeda, A., Teramoto, S.and Seki, T.: Proceedings of the International Conference on Food Factors : Chemistry and Cancer Prevention, Osawa, T.et al. (eds) Inhibition site of methyl allyl trisulfid, a volatile oil component of garlic, in the platelet arachidonic acid cascade.Springer-Verlag (in printing),
Description
「研究成果報告書概要(欧文)」より
