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1995 Fiscal Year Final Research Report Summary

VITAMIN E REQUIREMENT OF SEAWATER FISH LARVAE

Research Project

Project/Area Number 06660258
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionTOKYO UNIVERSITY OF FISHERIES

Principal Investigator

TAKEUCHI Toshio  TOKYO UNIVERSITY OF Fisheries, AQUATIC BIOSCIENCES,PROFESSOR, 水産学部, 教授 (70092591)

Project Period (FY) 1994 – 1995
Keywordsvitamin E / yellowtail / pacific cod / larvae / docosahexaenoic acid / rotifer
Research Abstract

This research is conducted to investigate the absorption of vitamin E (VE) in rotifer and seawater fish larvae such as yellowtail and Pacific cod. Different kinds of VE,such as free form and acetate form were used in this experiment.
At first, the accumulation of free-and acetate-form of VE was investigated using rotifer. We obtained linear curves as follows ; Free form of VE : y_1=0.001X_1^2+0.026X_1+0.435 (r^2=0.996) Acetate form of VE : y_2=0.077X_2+0.078 (r^2=0.966) y_<1,2>=VE content (mg) of rotifer, X_<1,2>=supplementation of VE (mg) to rotifer culture medium. This result showed that free-and acetate-form are no different in accumulation till 100mg/101 of VE supplementation. However, at a level of above 100mg of VE supplementation, free type of VE is more incorporated into rotifer than acetate type.
Next, dl-acetate, dl-free, and d-free forms of VE were added to rotifer medium, and yellowtail and pacific cod larvae fed these enriched rotifers. It was difficult for rotifer to convert acetate form to free form. Fish larvae can convert acetate form to be a free form of VE,but its absorption was low compared with free form. However, growth and survival rate of fish larvae were not different between these kinds of VE form.
Yuglena was also used for emulsified materials. It is suggested that VE in Yuglena did not accumulate in the body of the fish very much.
These observations are useful when considering the design of emulsified materials for live foods and micro-diet for fish larvae.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 竹内俊郎他4名: "DHA強化ワムシのマダラ仔魚に対する栄養価" 日本水産学会誌. 60. 641-652 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takeuchi et al.: "Nutritive value of DHA enriched rotifer for larval cod" Nippon Suisan Gakkaishi. 60(5). 641-652 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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