1995 Fiscal Year Final Research Report Summary
Variation of eicosanoid production by activation of Kupffer cells.
| Project/Area Number |
06660385
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | University of Osaka Prefecture |
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Principal Investigator |
SUGANO Tsukasa College of Agriculture, University of Osaka Prefecture, Professor, 農学部, 教授 (30081516)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Kazuhiro College of Agriculture, University of Osaka Prefecture, Assistant Prof, 農学部, 助手 (30192561)
OHTA Mitsuaki College of Agriculture, University of Osaka Prefecture, Associate Prof, 農学部, 助教授 (20134504)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Kupffer cell / Prostaglandin / Phospholipase A_2 / PMA / Thioacetamide / Cirrhosis |
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| Research Abstract |
Since phorbol ester mimics the actions of LPS,we investigated the metabolic alternation by zymosan-pellet after infusion of phorbol 12-myristate 13-acetate (PMA) in the perfused rat livers. PMA-infusion enhanced the production of Tx B_2 and PGD_2 by zymosan-pellet and arachidonic acid.
The response of hepatic macrophages and the effects of platelet-activating factor in perfused liver were studied during production of experimental liver cirrhosis induced by thioacetamide in female Sprague-Dawley rats. Within four weeks of thioacetamide administration (300mg/L drinking water) , an increase in hepatic macrophage population and enlargement in cell size preceded the alterations characteristic of cirrhosis. The amount of PGD_2 and TxB_3 in perfusate induced by platelet-activating factor were seven-and five-fold greater, respectively, in thioacetamide-treated than in control rats. Zymosan mimicked the effects of platelet-activating factor. These results are consisted with the hypothesis that hepatic macrophages and platelet-activating factor play important roles in the development cirrhosis induced by thioacetamide.
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