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1995 Fiscal Year Final Research Report Summary

gene expression induced by UV-B Light Study on gene expression induced by UV-B light

Research Project

Project/Area Number 06660425
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAKEDA Junko  Kyoto Univ.Agric. Chem. Instructor, 農学部, 助手 (30026421)

Project Period (FY) 1994 – 1995
KeywordsUV-B responsive element / Phenyl alamin ammonia-lyase / canot suspension cells / UV-B response / PAL gene / action spectrum / UV-B photoreceptor / gene expression
Research Abstract

(1) Carrot pal-10 full length promoter (-2335) fused to reporter gene (luciferase) was transiently transformed to carrot protoplasts by electroporation, and promoter activity (luciferase) induced by UV-B light was examined. Pal-10 full length promotor expression was UV-B specific and involvement of UV-B photoreceptor in regulation of pal-10 gene is satisfied as shown in intact cells.
(2) UV-B specific cis element was examined using various length promoter fragments fused to CaMV 35S core promoter (-90). Two elements were obtained between -395 and -190 and their consensus were T (A) XTCXCCAACCC (A).
(3) However, truncated promoter at -570, containing UV-B responsive cis-elements did not show UV-B response.
(4) Both UV-B responsive cis-element and up (-2335 to -570) and down (-145 to + 145) stream sequences are necessary for correct expression of pal-10 promoter.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Takeda et al: "Achlion spectre of phenglalanine ammona-lyase and chalcone synthase expussion in carrat cells suspension" Phyrologra Plantarum. 91. 517-521 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozaki & Takeda J: "Reyulation of plenylalanine ammoms-lyase gene in cenit suspension cuetured cells" Phant Cell. Tissne and organ aulture. 38. 221-225 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda et al: "Action spectra of phenylalanine ammonia-lyase and chalcone synthase expression in carrot cells in shspension" Physiol Plantarum. 91. 517-521 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozeki & Takeda: "Regulation of uhenyl alanine ammoma-lyasl gene in Carret suspension caltwred cells" Plant Cell, Tissue and Organe culture. 38. 221-225 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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