1995 Fiscal Year Final Research Report Summary
Basic study on neuronal conrol of heart
| Project/Area Number |
06670073
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | National cardiovascular center reseatch institute |
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Principal Investigator |
YAMAZAKI Toji National cardiovasular center research institute department of cardiac physiology, senior staff, 心臓生理部, 室長 (20116122)
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| Project Period (FY) |
1994 – 1995
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| Keywords | chromatogram / sympathetic nerves / dialysis / noradrenaline / neural control / heart |
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| Research Abstract |
1) The present study describes a high-performance liquid chromatographic-electrochemical detection (HPLC-ECD) system for routine measurement of the low levels of norepinephrine (NE) found in the myocardial interstitial space. In this system, an in vivo detection limit of 100 fg in a 50mul injection was achieved for NE.Using cardiac dialysis technique, 20mu dialysates were sampled from the myocardial interstitial space at 2-min intervals. The basal dialysate NE concentration was 16.6<plus-minus>4.0pg/ml. This low detection limit allowed the dialysate NE concentration to be monitored for dysfunction of the cardiac sympathetic nerve terminal. This system offers a new possibility for routine analysis of myocardial interstitial NE level
2) The present study describes a high-performance liquid chromatographic electrochemical detection (HPLC-ECD) system for monitoring of dihydroxyphenylglycol (DHPG) in the myocardial interstitial spsce. Using cardiac dialysis technique, 10mul dialysates were sampled from the myocardial interstitial space (1min-fraction), and directly injected to HPLC-ECD system. An in vivo detection limit of 100 fg in a 10mul injection was achieved for DHPG.The basal dialysate DHPG concentration was 181<plus-minus>46 pg/ml. This system offers a new possibility for monitoring of myocardial interstitial DHPG level
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