1995 Fiscal Year Final Research Report Summary
Molecular cloning of an insulin receptor tyrosine-kinase substrate-1 (IRS-1) associated molecule
| Project/Area Number |
06670153
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Jikei University, School of Medicine |
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Principal Investigator |
NISHIYAMA Masaki Jikei University, School of Medicine, Department of Biochemistry II,Asistant Professor, 医学部・生化学第2, 講師 (00180662)
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| Project Period (FY) |
1994 – 1995
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| Keywords | IRS-1 / anti IRS-1 monoclonal antibody / IRS-2 |
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| Research Abstract |
The following resalts were obtained in the project :
1. A number of monoclonal antibodies (MoAbs) against human IRS-1 were produced.
2. Using both MoAb and the recombinant IRS-1 tyrosine-phosphoryrated in vitro as probes candidate cDNAs of IRS-1 associated proteines were isolated from HeLa cell cDNA library.
3. One of candidated cDNAs, named HH109 has been analyzed and was found to be a novel gene.
4. In the IRS-1 gene deficient mice an additional insulin responsed tyrosine-phosphorylated protein (pp190) was detected. We showed that pp190 was recognized by anti IRS-1 MoAb, 6G5.
5. To isolate the cDNA of pp190 we srceened the expression library with the MoAb, 6G5 as a probe. And we showed that MoAb, 6G5 recognized IRS-2 which was identical molecule to pp190.
7. There was no sequence similarity between HH109 and IRS-2.
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