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1995 Fiscal Year Final Research Report Summary

Early diagnosis of borderline disease of mycosis fungoides using in situ PCR method

Research Project

Project/Area Number 06670202
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionSaitama Medical School

Principal Investigator

ARAI Eiichi  Saitama Medical School, Instructor, 医学部, 講師 (60167228)

Project Period (FY) 1994 – 1995
KeywordsPCR / In situ hybridization / T-cell receptor / gamma-chain / Paraffin section / Cutaneous lymphoma / Mycosis fungoides / Pseudolymphoma
Research Abstract

As a preliminary study in the first year, we modified the method of Algata et al. (Diagn Mol Pathol 3 : 275,1994) by setting nine and three primers into 12 and three segments of V_<gamma> and J_<gamma>-region, respectively. In addition, we developed a semi-nested PCR method setting new inner primers in the side of J_<gammas> for application to paraffin sections. As the result we had 15 positive out of 33 cases of cutaneous T-cell ylmphoma (CTCL). In the second year we identified in these postitive cases effective primer sets with which we performed PCR amplification of TCR-gamma chain on paraffin sections. Moreover, on the same section we made in situ demonstration using labelled oligonucleotide placed between 5'-primer and 3'-primer.Positive dots were seen in nuclei by DAB staining or alkaliphosphatase staining.
By electron microscopy, immnohistochimstry, and DNA analysis, we studied four CTCL cases which had been classified as pseudolymphomas because they regressed spontaneously. As the result we clarified that the spontaneous regression was induced by cataclysmic apoptosis (Majno : Am J Pathol 146 : 3,1995), although all these cases qualified histologically as malignant lymphomas and with Southerm blotting demonstration of clonal lymphocytic proliferation. Moreover we demonstrated the existence of imcomplete apoptosis in a case of infantile digital fibromatosis which did not regeress until 14 years of age.
In the next project we will try to increase sensitivity in detective the rearrangedTCR-gamma chain from minute areas in paraffin sections by PCR method using temperature gradient gel electropheresis (TGGE) and denaturing GGE.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 新井栄一: "皮膚T細胞性リンパ腫におけるT-cell receptor-γ鎖のバウフィン切片上での形態学的同定" Proceedings of the Japanese Camcer Association 54th Arnual Mseting. 557 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新井栄一: "自然消退を示し、apoptosisが証明されたcutaneous ron-epidermotrotropic T-cell Lymphomaの4例" 日本網内系学会会誌. 35. 128 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai Eiichi: "Role of apoptosis in spontaneous regression of peripheral T-cell Lymohoma in the skin or subcutis." Human Pathology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野峰生: "14歳まで消退しなかったと思われたin-fantile digital fibromatosisの1例." 形成外科. 38. 635-640 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新井栄一: "Histologically malignant,clinically hanign皮膚リンパ増殖性疾患.Non-epidernotropic typeの1例." 皮膚のリンフォーマ. 13. 82-85 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大林寛人: "皮膚に初発し再発時に眼球侵襲をみた lymphoplasmacytic/lymphoplasmacytoid lymphoma." 皮膚のリンフォーマ. 14. 144-147 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai E,Katayama I: "Detection of T-cell receptor-gamma chain in cutaneous T-cell lymphoma by morphological method on paraffin sections." Proceedings of the Japanese Cancer Association.54th Annual Meeting.557 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai E,Katayama I: "Role of apoptosis in sptaneous Regression of peripheral T-cell lymphoma arising in the skin or subcutis." Human Pathology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano M,Arai E,et al.: "Infatile digital fibromatosis that persisted for fourteen years : A case report." Keisei Geka (Japan). 38. 635-640 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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