1995 Fiscal Year Final Research Report Summary

xpression, regulation and poly morphism of the uxil geuls

Research Project

Project/Area Number	06670214
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Experimental pathology
Research Institution	Chiba university
Principal Investigator	SIMIZU Bunshiti Chiba University School of Medicine, Professor, 医学部, 教授 (10072894)
Co-Investigator(Kenkyū-buntansha)	SHIRASAWA Hiroshi Chiba University School of Medicine, Assistant, 医学部, 助手 (00216194)
Project Period (FY)	1994 – 1995
Keywords	nearoblastoma cells / differentiation / mxil
Research Abstract	The myc proto-oncogene family plays an important role in the control of cellular growth and fifferentiation. Mxil, one of the Max-associated proteins, has been known to have an antagonistic action on Myc activity. We have cloned and sequenced the murine Mxil gene encoding Mxil, one of Max-associated proteins. Murine and human sequences showed 87.9% nucleotide (nt) and 90.3% Amino acid (aa) sequence homology, whereas murine and zebra fish sequences showed 67.2% nt and 67.8% aa sequence homology. Using this Mxil cDNA,we analyzed the expression of the gene during differentiation of neuroblastoma cells. The mxil mRNA increased during growth inhibition and differentiation, and decreased with serum stimulation in mammal cell lines. We have also found an AAAAC polymorphic repeat in the 3' non-coding region of the human mxil cDNAs and a difference between the mxil mRNA half-lives in some different cell lines.