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1995 Fiscal Year Final Research Report Summary

In Situ Immunocytological and Molecular Pathological Analysis of the Lesions in the Target Organs of Chronic GvH Disease

Research Project

Project/Area Number 06670225
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionShimane Medical University

Principal Investigator

HARADA Takayuki  Shimane Medical University, Department of Pathology, Professor, 医学部, 教授 (90112135)

Project Period (FY) 1994 – 1995
KeywordsChronic GvH disease / T cell / Spleen / Thymus / Immunohistochemistry / Cytokine / Apoptosis / S phase cell
Research Abstract

Following results were obtained using mouse models of chronic GvHR and GvHD.
1.Chronic GvHR was induced by inoculating parental lymphoid cells into F1 hybrid mouse. Combination of ATL and ATH,which were congenic recombinant strains differing only in H-2I and S region from each other, was chosen to induce class II-GvHR.Selective activation against partner's alloantigen of graft CD4^+ T cells was the primary event of the GvHR and then led to concomitant activation of both graft and host cells. In particular combinations of strains differing whole MHC antigens, namely, DBA/2* (C57BL/6xDBA/2) F1 or BALB/c* (BALB/cxA/J) F1, preferential but not selective activation of graft CD4^+ cells was characteristically observed. Contributing factors to this phenomenon were low responsiveness of graft CD8^+ T cells to allogeneic class I MHC antigens and anti-DBA/2 activity of host CD8 cells in the case of D2-GvHR.Predominant activation of CD4 cells in the chronic GvH-spleen was expressed as the ratio of CD4/CD8 was always >1.
2.Moderate atrophy of the thymus was observed in the 2nd week of GvHR.Increase of the frequency of apoptotic cell and decrease of S phase cell were cellular event leading to its atrophy. Although it was not so obvious as acute GvHR,cellular change occurred as early process of atrophy was essentially same between chronic and acute GvHR's.
3.Non-suppurative destructive cholangitis was a liver lesion of chronic GvH disease. It was characterized by periductal and intraepithelial infiltration of lymphocyte in the portal area and strong expression of MHC class II antigen on the epithelial membrane as early as 2nd day of GvHR.Interferon-gamma produced in site was important factor to induce aberrant expression of the antigen.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 原田,孝之: "移植の合併性-GvHD" 病理と臨床. 11. 443-450 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada,Takayuki: "Graft-Versus-Host Reaction and GvH disease" Tohoku Journal of Experimental Medicine. 173. 171-181 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和田,昌弘: "抗原処理と提示に対する抗原性状の反映" Annual Review 免疫1995. 54-61 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 原田,孝之: "骨髄移植をその発展" 島根医学. 16. 11-19 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 原田,孝之: "骨髄移植" 臨床免疫学,狩野他編集,朝倉書店. 印刷中. (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada, T.: "Complications in transplantation-GvHD." Pathology and Clinic (Byori to Rinsho, in Japanese). 11. 443-450 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, T.: "Graft-versus-Host Reaction and GvH disease." Tohoku Journal of Experimental Medicine. 173. 171-181 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wada, M., and Harada, T.: "Antigen processing and presentation in relation to antigen nature." Annual Review in Immunology, 1995 (Annual Review Men'eki 1995 in Japanese). 54-61 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, T.: "Progress in Bone Marrow Transplantation." Shimane Journal of Medicine (Shimane Igaku in Japanese). 16. 11-19 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, T.: Bone Marrow Transplantation.Clinical Immunology (Rinshomen'ekigaku in Japanese) ed.by Kano et al.Asakura-Syoten, Tokyo (in press), (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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